Instructions for Use - Glyco Kit MB-LAC Con A - Bruker

Intended Use
The Glyco Kit MB-LAC Con A (Magnetic Bead-Lectin Affinity Chromatography with Concanavalin
A) is developed for specific capturing of N-glycosylated proteins and peptides derived from
biological samples based on lectin affinity prior to MALDI-TOF mass spectrometry analysis by
means of e.g. Bruker´s microflex ® , autoflex ® or ultraflex ® MALDI-TOF.
This kit is for research use only. It is not for use in diagnostic procedures.
Product description
The MB-LAC Con A kit is designed for enrichment and purification of N-glycosylated peptides and
proteins prior to MALDI-TOF MS analysis. The MB-LAC Con A kit is based on super-paramagnetic
microparticles functionalized with Concanavalin A.
Concanavalin A is a lectin which specifically binds glucose and/or mannose residues of
polysaccharides and glycoprotein/-peptides containing unmodified hydroxyl groups at positions C3,
C4 and C6 6 , 7 , 8 . The binding motif is depicted in Fig. 3. The green highlighted mannose residues of
the pentacore of N-glycosylated peptides/proteins interact with Concanavalin A.
MB-LAC
Con A
Glycoprotein
Man
GlcNAc
Fig. 3: Pentacore of N-glycosylated peptides/proteins. Mannose
residues responsible for the interaction with Concanavalin A
are highlighted in green.
Due to the binding requirements ConA binds N-glycans of the high mannose type, the hybrid type
and bi-and tri-antennary complex type. According to this, it can be applied as a general tool for the
capturing of N-glycosylated peptides and proteins with broad specificity.
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