Instructions for Use - Glyco Kit MB-LAC Con A - Bruker

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Cleaning of polished steel targets This is an advanced cleaning protocol differing from the standard cleaning procedure recommended in the respective target’s product description. 1. Rinse the target intensively under flowing hot tap water. Hot tap water 2. Wipe the target intensively with acetone using a kimwipe. : Acetone is highly flammable and irritant (R: 11-36-66-67; S: (2)-9-16-26) Acetone 3. Rinse the target intensively under flowing hot tap water. Hot tap water 4. Wipe the target intensively with 80% TFA using a kimwipe. : 80% TFA is corrosive (R: 20-35- 52/53; S: (1/2)-9-26-27-28-45-61) 80 % TFA 5. Rinse the target intensively under flowing hot tap water. Hot tap water 6. Rinse the target with distilled water (Milli-Q). Distilled water 7. Rinse the target with methanol and let it dry. : Methanol is highly flammable and toxic (R: 11-23/24/25-39/23/24/25; S: (1/2)-7-16-36/37-45) Methanol Page 12 of 21
Matrix preparation on the MALDI-MS targets Analysis of glycoproteins using 2,5-DHAP For preparation of glycoproteins within the mass range of 6 – 100 kDa on AnchorChip TM 600 targets (#209513) we recommend the use of 2,5-dihydroxyacetophenone (2,5-DHAP, #231829 or #231830) as matrix solution. 2,5-DHAP preparation on AnchorChip600 targets 1. Dissolve 7.6 mg DHAP in 375 µl ethanol. : DHAP is irritant (R: 36/37/38; S: 26-28-36/37-60), ethanol is highly flammable (R: 11; S: (2)-7-16) 7.6 mg DHAP 375 µl ethanol 2. Add 125 µl (18 mg/ml in Milli-Q water) of a di-ammonium hydrogencitrate solution (= 10 µmol). : Diammonium hydrogencitrate is irritant (R: 36/37; S: 26) 125 µl Diammonium Hydrogen Citrate 3. Vortex for 1 min at RT. Vortex 4. Sonicate for 15 min. Sonificate 5. Vortex for 1 min at RT. The matrix solution is stable for at least one week when kept in the dark Vortex 6. Mix 2 µl of the sample with 2 µl 2 % TFA and subsequently add 2 µl matrix solution. : 2% TFA is irritant (R: 36/38; S: 26- 28-37-60) 2 µl Sample 2 µl 2% TFA 2 µl DHAP Matrix Solution 7. The mixture of sample, TFA and DHAP has to be vigorously mixed by pipetting up and down. It is important that the solution becomes turbid before spotting Vigorously mixing Page 13 of 21
Page 1 and 2: CARE products are designed for supp
Page 3 and 4: Table of Contents General Informati
Page 5 and 6: Kit Components Materials provided i
Page 7 and 8: Recommended sample preparation proc
Page 9 and 10: 14. Transfer the tube from the Magn
Page 11: MALDI-TOF MS Target Preparation MAL
Page 15 and 16: 7. After drying of the spots the ta
Page 17 and 18: Examples Isolation of a small glyco
Page 19 and 20: References 1 Sparbier K, Koch S, Ke
Page 21: Ordering Information Product Size (

Instructions for Use - Glyco Kit MB-LAC Con A - Bruker

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