Instructions for Use - Glyco Kit MB-LAC Con A - Bruker

6. Repeat steps 3 and 4 to wash the beads and
remove the supernatant
Repeat steps 3 and 4
7. Re-suspend the beads in 10 µl BB 1 (for
glyco-proteins) or BB 2 (for glyco-peptides)
and add up to 10 µl of the sample. If you have
less sample volume adjust to 10 µl by adding
deionized water.
For the analysis of serum, use 10 - 20 µl
serum. Firstly, pipette an equal amount of BB
1 or BB 2 (10 - 20 µl) to 20 µl magnetic beads
and subsequently add the serum.
10 µl BB1 or BB2
10 µl sample
8. Incubate the magnetic beads for 1 h at room
temperature while swirling the tube from time
to time to mix the beads.
1 h incubation
Washing of beads
9. Place the tube on the Magnetic Separator and
wait for 20 seconds to separate the beads
from the supernatant. (Note the movement of
the magnetic beads in the tube.)
Collect beads
10. Remove the supernatant carefully. Avoid
contact of pipette tips with the magnetic beads
and take care not to remove the beads.
Remove supernatant
11. Add 100 µl WB 1.1 (for glyco-proteins) or
WB 2.1 (for glyco-peptides).
100 µl WB1.1 or WB2.1
12. Move the tubes back and forth between
adjacent wells at least 20 times to wash the
beads.
Move back and forth
13. Wait 20 seconds for collecting magnetic beads
at the wall of the tubes and remove the
supernatant carefully.
Collect beads
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