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MFP-3D Procedural Operation ‘Manualette’ Version 10 (v080501; Igor 6.04A) ii 1. Introduction to the MFP-3D 3D 2. Opening the MFP-3D 3D software 3. Loading the Probe 4. Aligning the Laser 5. Thermal Tuning 6. AC mode Operation: 6.1 in Air; 6.2 In Fluid 7. Managing Image Windows 8. Contact Mode Operation 9. Spring Constant Determination 10. Basic MicroAngelo use 11. Basic Force Spectroscopy 12. Introductory Data Analysis 13. ARgyle 3D Image Rendering 14. Misc. Procedures 16. Noise Measurements 17. RF Quick Reference
Ryan’s MFP-3D Procedural Operation ‘Manualette’ Version 10 (v080501; Igor 6.04A); ix The purpose of this ‘Manualette’ is to offer new and infrequent MDP-3Dusers some supplemental documentation on how to procedurally operate an Asylum Research MFP-3D AFM. It’s like a manual, but more brief and to the point regarding basic ‘how-to’ operational procedures. It started off as a small document, and was termed the manualette. Its inception was to alleviate time spent training lab members during the author’s post doc days, but has evolved to this document in efforts to reduce the operational learning curve to achieve your efficiency. Also note that this instrument’s standard software is highly versatile, and describing even the most straightforward techniques in our user base is quite an undertaking. Adding the openness of the software, MFP- 3D accessories (heaters, ORCA, VFM, etc.) and signal routing with the crosspoint switch, makes the instrument mind-numbingly versatile, and there may be a good chance it’s not described. It is assumed that the official MFP-3D manual has been perused. The MFP-3D is an expensive, high precision instrument, and should be treated with great care and common sense. AFM does take patience since it is an instrument that must be tuned and have proper sample preparation to be successful. A great effort has been made to ensure this manualette is as accurate as possible, but its accuracy and completeness cannot be guaranteed. Despite the best efforts by the author, this manualette may contain mistakes and the reader should use this only as a general guide for operation of the MFP-3D atomic force microscope, and is not the ultimate source of information about this instrument. The author assumes no responsibility for any damage whatsoever by persons or property that may result from readers undertaking operation of the MFP-3D. This Manualette: • Is broken down into sections that are in a (seemingly) logical progression for obtaining AFM images or simple force spectroscopy experiments, and the subsequent (basic) data analysis. • Only discusses operation of the MFP-3D itself, and does not include any optical microscopy techniques (if your MFP-3D happens to be sitting on an inverted optical microscope), or operation of electrical techniques or accessory modules at this time. • Is in continual development, so more insightful, updated versions will occur in the future. Version 10 by far is the largest and most comprehensive to date. If you are not familiar with Igor Pro, you should read through the ‘Getting <strong>St</strong>arted’ manual that comes with the software documentation. It’s a worthwhile use of a couple hours. The author also suggests when updating new software from Asylum, to read the ‘what’s new read me’ files; they are often very descriptive. The ‘MFP3D Help Files’ under the Igor Help menu are also very useful, especially for image analysis and display. The programmers write these for a reason, and are generally very insightful, yet sometimes not procedural- hence, the manualette. Some features of this manualette- -Any word in gray bold text in single quotes means it’s an execution button in the software; example ‘Simple Engage’, represents this button: The ▪ bullet points denote a new concept or comment. ?
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B - St. Cloud State University

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