B - St. Cloud State University

More documents

Recommendations

Info

Ryan’s MFP-3D Procedural Operation ‘Manualette’ Version 10 (v080501; Igor 6.03A); ©2008 6.20 •The same attractive / repulsive imaging mode rules seem to apply to the second mode as does the first. The author suggests using the protocols described in Section 6.1.4 to avoid mode hoping in the second mode. • For this protocol, the author has found that some water based latex paint gives excellent contrast in the Amplitude & Phase images, and can easily be made by most people wanting to practice/ learn how to do this technique. The sample is a small amount of Sherwin-Williams ProMar200 water based latex on a glass slide (as a former professional carpenter, the author’s of the opinion that Sherwin Williams is one of the best paintson the market). Water based paints are heterogeneous because of the small emulsion polymer particles and other fillers used as the ingredient that gives the paint its opaque character. For in-air DualAC imaging- To get started, determine the fundamental resonant frequency of the cantilever (if not known) by performing a thermal tune (see Section 5); to determine the second eigenmode, roughly multiple by 6.1- this will be important for entering high and low tune values for the DualAC Auto Tune. An example of a thermal tune of an Olympus AC 240 silicon cantilever (f~70kHz; k~2N/m) is shown in Figure 6.1.5.2. Notice the second ‘eigenmode’ is roughly 6 times the fundamental frequency (inset). Figure 6.1.5.2: Thermal tune showing fundamental and second eigen mode peaks Confirm that Height, Phase1, Amplitude1, Phase 2 and Amplitude2 are the activated image channel tabs in the Master Channel Panel (see Section 7). Go to the Tune tab of the Master Panel. Click on the DualAC Mode check box in the ‘Other Things’ portion of the panel; this will expand the panel to include the second mode parameters (Figure 6.1.5.3). Confirm that the Auto Tune ‘High’ and ‘Low’ setvar values are in the range of the cantilever being used.
Ryan’s MFP-3D Procedural Operation ‘Manualette’ Version 10 (v080501; Igor 6.03A); ©2008 6.21 Choose a Target Percent and Target Amplitude for the fundamental resonant peak (i.e., first eigen mode)- The author usually goes with 1V and -5% to be in repulsive mode (see Section 6.1.4). Click on ‘Auto Tune’ in the first column (left) which is for the the fundamental frequency of the cantilever. This will tune the cantilever is it normally does in standard AC imaging (see Section 6.1.1). For second mode tuning- Confirm that the ‘Auto Tune High’ and ‘Low’ setvar values will be sufficient to include the desired second mode frequency. Choose a Target percent and Target Amplitude for the second mode- The author usually goes with the default 100mV and -5% to be in repulsive mode. Click the ‘Auto Tune’ Button; after a moment, the software should choose a frequency. • Notice the Frequency Ratio setvar value is updated to the multiplier value of the fundamental peak. NOTE: If the Auto Tune for the second mode doesn’t happen to be successful, in certain instances the coupling of the probe chip to the cantilever holder may be too good, causing the Drive Amplitude to be too small to achieve the defined Target Amplitude- when this happens, increase the target amplitude and step it down later once imaging commences. Perform a ‘gentle engage’ as described in Section 6.1.2. once the tip is hard engaged on the surface, adjust the Drive amplitude appropriately to keep it in the imaging mode desired (i.e., repulsive or attractive). Figure 6.1.5.3: Expanded Tune tab for Dual AC. <strong>St</strong>art imaging. Notice that the Main tab of the Master Panel now has an expanded panel offering Drive Amplitude and Drive Frequency setvars with radio button for image tuning. Save the data if you want.
Page 1 and 2: Atomic Force Microscopes Santa Barb
Page 3 and 4: Ryan’s MFP-3D Procedural Operatio
Page 45: Ryan’s MFP-3D Procedural Operatio
Page 97 and 98:
Ryan’s MFP-3D Procedural Operatio
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
Page 237 and 238:
Page 239 and 240:
Page 241 and 242:
Page 243 and 244:
show all

B - St. Cloud State University

Create successful ePaper yourself

Delete template?

Save as template?