Internal Standard Application to Dried Blood Spots ... - lc-ms.de
Internal Standard Application to Dried Blood Spots ... - lc-ms.de
Internal Standard Application to Dried Blood Spots ... - lc-ms.de
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Variability of IS Peak Area After Extraction & LC-MS/MS<br />
• Tested with a pro<strong>to</strong>type sprayer, not optimized for DBS (EBF Poster 2010)<br />
• IS-mix solution either<br />
- sprayed on<strong>to</strong> neat card before blood spotting<br />
OR<br />
- sprayed on<strong>to</strong> dried blood spot<br />
• Extraction with DBS-MS 16 pro<strong>to</strong>type, Analysis by LC-MS/MS<br />
Means N = 4<br />
IS sprayed before blood<br />
spotting<br />
IS sprayed on<strong>to</strong> dried<br />
blood spot<br />
Card 1<br />
Card 2<br />
Card 1<br />
Card 2<br />
NOR-D3<br />
Mean Area<br />
x103 x103 814<br />
815*<br />
3229<br />
2763<br />
CV [%]<br />
12.8*<br />
*N=3: data of 4th spot consi<strong>de</strong>red as outlier, since IS solution was run out.<br />
• CVs of peak areas of extracted IS<br />
- 0.5 – 7.3% if IS sprayed on<strong>to</strong> dried blood<br />
- 5.6 – 6.9% if IS sprayed on<strong>to</strong> neat card<br />
- Precision of 15 % (20% @ LLOQ) accepted in Bioanalysis<br />
• More <strong>de</strong>tailed investigations are ongoing<br />
6.8<br />
5.8<br />
0.5<br />
Mean Area<br />
x103 x103 367<br />
370*<br />
1011<br />
789<br />
LOR-D5<br />
CV [%]<br />
5.6<br />
16.7*<br />
7.3<br />
4.6<br />
Mean Area<br />
x103 x103 76<br />
72*<br />
250<br />
202<br />
FOR-D6<br />
CV [%]<br />
6.9<br />
10.0*<br />
4.7<br />
3.0
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