Internal Standard Application to Dried Blood Spots ... - lc-ms.de
Internal Standard Application to Dried Blood Spots ... - lc-ms.de
Internal Standard Application to Dried Blood Spots ... - lc-ms.de
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Homogeneity of Spraying on HPTLC Plates<br />
For equipment validation in HPTLC the following is done<br />
• 0.5 mg/mL Oracet Red G [1-(methylamino)anthracene-9,10-dione] in methanol<br />
used as a test dye for spraying<br />
• Spraying with the au<strong>to</strong>mated high precision sprayer ATS4 (CAMAG)<br />
• Merck 60 F254 HPTLC plates <strong>to</strong> measure the intensity variation of the<br />
spraying process<br />
• Analysis with an optical scanner (CAMAG TLC Scanner 4), <strong>de</strong>tection<br />
collima<strong>to</strong>r 4 x 0.3mm (length x width) in remission mo<strong>de</strong> at 546 nm<br />
A similar process was used for DBS, but with 14C coffeine mimicking an IS, and<br />
<strong>de</strong>termination by Imaging like QWBAR<br />
ATS4 HPTLC Sprayer DBS-MS 500 Sprayer
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