Internal Standard Application to Dried Blood Spots ... - lc-ms.de
Internal Standard Application to Dried Blood Spots ... - lc-ms.de
Internal Standard Application to Dried Blood Spots ... - lc-ms.de
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Introduction<br />
• IS are ad<strong>de</strong>d in the extraction solvent for DBS analysis so far<br />
– IS not in liquid blood, nor penetrating in<strong>to</strong> dried blood<br />
– IS does not compensate for losses during sample preparation<br />
– IS compensates for matrix effects (SIL) and injection proble<strong>ms</strong><br />
• Alternative methods for IS addition are <strong>de</strong>sirable, <strong>to</strong><br />
– Mimick all effects acting on analyte as close as possible<br />
– Result in IS recovery similar <strong>to</strong> analyte<br />
• “IS Addition” subteam formed within the EBF “DBS <strong>to</strong>pic team”<br />
• Addition of IS by spraying was evaluated
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