Internal Standard Application to Dried Blood Spots ... - lc-ms.de
Internal Standard Application to Dried Blood Spots ... - lc-ms.de
Internal Standard Application to Dried Blood Spots ... - lc-ms.de
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Spraying with ATS4 (Au<strong>to</strong>matic TLC Sampler, CAMAG)<br />
• [1-Methyl- 14 C] Caffeine, MW 194.2 g/mol, mimicking an IS<br />
H 3<br />
C*<br />
O<br />
N<br />
O<br />
N<br />
CH 3<br />
N<br />
N<br />
CH 3<br />
• Specific Activity: 55 mCi/mMol = 11241 kBq/mg<br />
• Concentration in ethanol: 0.1 mCi/mL = 3.7 MBq/mL = 222 x 106 dpm/mL<br />
• Spray Solution: Target 14C concentration of 40000 dpm/20 µL<br />
• 60 µl [1-Methyl- 14 C] Caffeine <strong>Standard</strong> in 6 mL Methanol<br />
• Concentration measured: 3 Aliquots a 20 µl = 39448 dpm ± 0.9 %<br />
corresponding <strong>to</strong> 98.6% of Target<br />
• 10 x 10 mm area microsprayed (5 – 40 µL) covering the whole blood<br />
spot (see figures)
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