Internal Standard Application to Dried Blood Spots ... - lc-ms.de
Internal Standard Application to Dried Blood Spots ... - lc-ms.de
Internal Standard Application to Dried Blood Spots ... - lc-ms.de
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Conclusion<br />
• The maximum capacity of the filter paper was < 40 µL of spray solution /cm 2<br />
• Overfilling leads <strong>to</strong> uncontrolled spreading and higher variability, explaining<br />
the higher CV at 40 µL spray volume<br />
• Recommen<strong>de</strong>d spray application volumes are 5 - 20 µL/cm2 • The homogeneity within the 4 mm extraction area of the blood spot was high<br />
• Precision and accuracy of the spray process were high<br />
• The imaging analysis with 14C Caffeine confirmed that the blood spot absorbs<br />
the sprayed solution homogeneously<br />
• Boundary effects appeared outsi<strong>de</strong> the 4 mm region which is extracted<br />
• Spray-drift of IS in<strong>to</strong> adjacent blank spots was well below 1 %<br />
• CVs of areas of extracted IS were 0.5 – 7.3% (N=3-4) with pro<strong>to</strong>type sprayer<br />
• Spraying of IS is a very good alternative <strong>to</strong> addition of IS in extraction solvent
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