You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
<strong>EMBL</strong> Research at a Glance 2009<br />
Advanced Light Microscopy Core Facility<br />
Rainer<br />
Pepperkok<br />
PhD 1992, University<br />
Kaiserslautern.<br />
Postdoctoral research at<br />
University of Geneva.<br />
Lab Head at the Imperial<br />
Cancer Research Fund,<br />
London.<br />
At <strong>EMBL</strong> since 1998.<br />
The Advanced Light Microscopy Facility (ALMF) offers a collection of state-of-the-art light microscopy<br />
equipment and image processing tools. The ALMF was set up as a cooperation between<br />
<strong>EMBL</strong> and industry to improve communication between users and producers of high-end microscopy<br />
technology and to support in-house scientists and visitors in using light microscopy<br />
methods for their research. The facility also organises regularly in-house and international courses<br />
to teach advanced light microscopy methods.<br />
Major projects and accomplishments<br />
• The ALMF presently manages 18 top-of-the-line microscope systems and five image<br />
analysis workstations from leading industrial companies.<br />
• More than 30 visitors per year come to carry out their own experiments in the ALMF or<br />
to evaluate microscopy equipment.<br />
• The ALMF was the seed for the European Light Microscopy Initiative (ELMI) that<br />
establishes links with light microscopy facilities throughout Europe.<br />
• Usage of the facility has reached 30,000 hours per year.<br />
Services provided<br />
• Assisting all <strong>EMBL</strong> groups and visitors through the microscopic imaging process: project planning, sample preparation, staining,<br />
microscope selection and use, image processing, presentation, data transfer and storage.<br />
• Developing accessory software and microscopy equipment, co-developments with industrial partners, pre-evaluation of commercial<br />
equipment.<br />
Technology partners<br />
The ALMF presently has collaborations with the following companies:<br />
• Applied Precision*<br />
• Bitplane*<br />
• Carl Zeiss*<br />
• Cell Biotrading*<br />
• Lambert Instruments*<br />
• Leica Microsystems*<br />
• Olympus Europe*<br />
• Perkin Elmer*<br />
• Scientific Volume Imaging<br />
• Visitron*<br />
* The ALMF also serves as a reference site for these companies.<br />
Selected references<br />
Simpson, J.C., Cetin, C., Erfle, H., Joggerst, B., Liebel, U., Ellenberg,<br />
J. & Pepperkok, R. (2008). An RNAi screening platform to identify<br />
secretion machinery in mammalian cells. J Biotechnol., 129, 352-65<br />
Brunet, S., Zimmermann, T., Reynaud, E.G., Vernos, I., Karsenti, E. &<br />
Pepperkok, R. (2006). Detection and quantification of proteinmicrotubules<br />
interactions using green fluorescent protein<br />
photoconversion. Traffic, 7, 1283-9<br />
Pepperkok, R. & Ellenberg, J. (2006). High-throughput fluorescence<br />
microscopy for systems biology. Nat. Rev. Mol. Cell Biol., 7, 690-6<br />
Zimmermann, T., Rietdorf, J. & Pepperkok, R. (2003). Spectral<br />
imaging and its applications in live cell microscopy. FEBS Lett., 56,<br />
87-92<br />
56
Change language