Model Organisms in Drug Discovery

Contents
List of contributors ...................................... xi
Acknowledgments ....................................... xiv
1 Introduction to Model Systems in Drug Discovery
Kevin Fitzgerald and Pamela M. Carroll .................... 1
1.1 Integrating model organism research with drug discovery. . . 1
2 Growing Yeast for Fun and Profit: Use of Saccharomyces cerevisiae
as a Model System in Drug Discovery
Petra Ross-Macdonald ................................. 9
2.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.2 Saccharomyces cerevisiae and its genome: a brief primer. . . . 10
2.3 Yeast in pathway and mechanism elucidation . . . . . . . . . . . 13
2.4 An example of mechanism elucidation:
immunosuppressive agents . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.5 Application in pathway elucidation: G-protein-coupled
receptor/mitogen-activated protein kinase signaling . . . . . . . 17
2.6 Applications in pathway deconstruction/reconstruction . . . . 18
2.7 Applications to the study of protein function . . . . . . . . . . . . 19
2.8 Reagents and resources available in yeast. . . . . . . . . . . . . . . 22
2.9 Gene expression profiling using microarrays. . . . . . . . . . . . . 24
2.10 Deletion collections: reinventing traditional screens . . . . . . . 25
2.11 Overexpression analysis: enough is enough. . . . . . . . . . . . . . 29
2.12 Proteomics: would you like chips with that?. . . . . . . . . . . . . 29
2.13 Web-accessible databases: bringing it all back home. . . . . . . 31
2.14 Conclusion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
2.15 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
3 Caenorhabditis elegans Functional Genomics in Drug Discovery:
Expanding Paradigms
Titus Kaletta, Lynn Butler and Thierry Bogaert .............. 41
Model Organisms in Drug Discovery. Edited by Pamela M. Carroll and Kevin Fitzgerald.
& 2003 John Wiley & Sons, Ltd. ISBN 0 470 84893 6