Biophysical studies of membrane proteins/peptides. Interaction with ...

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ABBREVIATIONS AND SYMBOL LIST ABBREVIATIONS AND SYMBOL LIST C cmc C p D DLPC DMPC DMPE DMPA DOPC DOPG DPPC DSC DSPC ∆G 0 ESR F GUV H0-NBAR H I H II k K p K b L L i L α L β L β’ L c Specific heat Critical micelle concentration Heat capacity at constant pressure Lateral diffusion coefficient 1,2-Dilauroyl-sn-glycero-3-phosphatidylcholine 1,2-Dimyristoyl-sn-glycero-3-phosphatidylcholine 1,2-Dimyristoyl-sn-glycero-3-phosphatidylethanolamine 1,2-Dimyristoyl-sn-glycero-3-phosphatidic acid 1,2-Dioleoyl-sn-glycero-3-phosphatidylcholine 1,2-Dioleoyl-sn-glycero-3-phosphatidylglycerol 1,2-Dipalmitoyl-sn-glycero-3-phosphatidylcholine Differential scanning calorimetry 1,2-Distearoyl-sn-glycero-3- phosphatidylcholine Free energy <strong>of</strong> binding Electron spin resonance Lipid perturbation energy Giant unilamellar vesicle N-terminal amphipatic helix <strong>of</strong> N-BAR domain Hexagonal type I phase Hexagonal type II (or inverted) phase Boltzmann constant Partition coefficient Binding constant Lipid phase Lipid species i Fluid liquid-disordered phase Gel solid-ordered phase Tilted gel phase Crystalline phase ix
Page 1: UNIVERSIDADE TÉCNICA DE LISBOA INS
Page 4 and 5: Fernandes, F., Loura, L. M. S., Fed
Page 6 and 7: Ao Pedro e Hugo, amigos de longa da
Page 8 and 9: 2.2. - Peptides as models 2.3. - Am
Page 12 and 13: L o L/P LUV MARCKS mcp MLV n S,i PI
Page 15: ABSTRACT ABSTRACT Biomembranes are
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INTRODUCTION: LIPID-PROTEIN INTERAC
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particle is 900 nm in length and 6.
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thickness than mcp, the N-terminus
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DEPENDENCE OF M13 MCP OLIGOMERIZATI
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M13 Coat Protein Lateral Distributi
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344 Biophysical Journal Volume 87 J
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346 Fernandes et al. THEORETICAL BA
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348 Fernandes et al. FIGURE 3 Donor
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350 Fernandes et al. efficiencies (
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352 Fernandes et al. Lehtonen, J. Y
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central D-subunit, which in turn dr
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Our understanding of the mechanism
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Biochemistry 2006, 45, 5271-5279 52
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V-ATPase Indole Inhibitor Interacti
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Biochimica et Biophysica Acta 1758
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F. Fernandes et al. / Biochimica et
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BINDING OF A QUINOLONE ANTIBIOTIC T
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[ 1+ (2 / π)arcsin( R' / 2R )] BIN
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INTERACTION OF HELIX-0 OF THE N-BAR
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Abstract A group of proteins with c
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Methods Materials Peptides H0-NBAR,
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is the result of the immobilization
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where τ 0 is the lifetime of the d
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at very high concentrations, confir
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References 1. Farsad, K., and P. De
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TABLE I Membrane/Water partition co
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Fig. 7: H0-NBAR increases phospholi
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FIG. 2 18
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FIG.4 20
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Fig. 5B 22
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FIG.7A FIG. 7B 24
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CLUSTERING OF PI(4,5)P 2 IN FLUID P
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Absence of clustering of phosphatid
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where I is the fluorescence intensi
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certainly feasible that PI(4,5)P 2
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the tilt angle, and maintain a larg
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Chapter III ‐ Binding of Inhibito
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Chapter V - Interaction of Helix‐
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188
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Some of the biological questions de
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Bowman, E. J., Graham, L. A., Steve
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Farsad, K., Ringstad, N., Takei, K.
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Hinderliter, A., Biltonen, R. L., a
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Lodish, H., Berk, A., Zipursky, S.
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Nikura, K., Takeshita, N., and Taka
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Rodan, G. A. and Martin, T. J. (200
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J. Biol. Chem. 270: 17934-17938. Te
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