Here - Stiftung Forschung 3R

Respiratory Skin Controls
Protease 1 *
Protease 2 *
Chemicals – Pre/Prohaptens
Proteins (N = 1)
(N = 4)
Protease 3 * Isoeugenol (Iso) Human serum albumin
(HSA)
Protease 4 *
Eugenol (Eug)
Protease 5 *
Cinnamic alcohol (CA)
Protease 6 *
Paraphenylenediamine
(PPD)
Xylanase 1 *
Xyloglucanase 1 *
considered.
Exclusion criteria included the following:
• Gases and highly volatile chemicals
• Insoluble chemicals
• Metals
• High molecular weight chemicals.
With regard to reference data sources for
the chemicals selection, two sources were
mainly taken into consideration: the LLNA
database (Gerberick GF et al., 2005) and
the European Centre for Ecotoxicology
and Toxicology of Chemicals Technical
report N o .77 (ECETOC; 1999) and N o .87
(ECETOC; 2003). [Complete references
not supplied]
2. Proteins
The selection of the proteins to be used
for the development of the tests has
been performed according to predefined
criteria.
Inclusion criteria included the following:
1) Proteins should be backed by the
availability of high quality in vivo data.
2) Proteins should be readily available.
3) Proteins should cover a relevant range
of protein classes.
Reference data sources: Protein selection
was based upon historical human and
animal data from Novozymes AS (industrial
enzymes) and the Allergome Platform for
Allergen Knowledge (allergome.org).
Toolbox
The Sens-it-iv toolbox is the major
deliverable of the project and its ultimate
legacy after its official termination on
31 March 2011. It contains those assays
developed within the Sens-it-iv project,
or the Sens-it-iv sphere with contribution
of Sens-it-iv, which the consortium found
to be worthy of the search for financial
support beyond the end of the project.
In contrast to the LLNA, these in vitro
tests are usually exclusive for skin or
PROGRESS REPORTS FROM EU-FUNDED PROJECTS
Progress Report 2011 & AXLR8-2 Workshop Report
165