Here - Stiftung Forschung 3R

Figure 3. The Sensor Dish Reader (SDR) system from partner PreSens GmbH. This optical
fluorescence-based sensor technology allows for optical on-line respiration measurements and
monitoring of pH in the cell cultures. Monitoring of the important physiological parameters pH
and oxygen is essential for assessing the physiological condition of a cell culture. Consequently,
this also becomes an efficient means for detection of toxic effects on the culture. Optical microsensors
for pH and oxygen offer many advantages over other sensor systems, such as non-invasive
and non-destructive measurement from the outside, through the transparent wall of a small
bioreactor or cell culture plate. Optical sensors can also easily be combined with other biosensor
methods. The panels show culture plates placed in a cell culture incubator for on-line monitoring;
one unit of culture well with fluorescence spot; SDR plate optimised for reduced size wells.
of reference compounds the cells are
characterised and ranked for suitability in
safety screening.
An aluminum holder for small plastic
vessels (from 96-well strip plates)
with integrated optical oxygen sensors
were tested and evaluated for use with
the SensorDish Reader for respiration
measurements (Figure 3). Thus, less
sample volume is necessary, which
significantly reduces the numbers of
cells needed for detection of the oxygen
consumption. For homogenisation of
the oxygen ingress in the 24 wells, a
cover clamp system (from Applikon) was
modified for use with the SensorDish
Reader. The homogeneity of the oxygen
ingress into the wells using this system
was tested successfully with the sodium
sulphite method.
Publications 2010-11
1. Mandenius CF, Steel D, Noor F, et al. Cardiotoxicity testing using pluripotent stem
cell-derived human cardiomyocytes and state-of-the-art bioanalytics: a review. J
Appl Toxicol. 2011; 31, 191-205.
2. Asp J, Steel D, Jonsson M, et al. Characteristics of cardiomyocyte clusters derived
from human embryonic stem cells: Effect of in vitro maturation and comparison
with human heart tissue. J. Mol. Cell. Biol. 2010; 2, 276-83.
3. Andersson H, Kågedal B, Mandenius CF. Surface plasmon resonance measurement
92 PROGRESS REPORTS FROM EU-FUNDED PROJECTS
Progress Report 2011 & AXLR8-2 Workshop Report