April Journal-2009.p65 - Association of Biotechnology and Pharmacy
April Journal-2009.p65 - Association of Biotechnology and Pharmacy
April Journal-2009.p65 - Association of Biotechnology and Pharmacy
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong><br />
Vol. 3 (2) 210-218, <strong>April</strong> 2009. ISSN 0973-8916<br />
212<br />
was carried out. The predicted structure contains<br />
a fold (â/á) 8<br />
shape at the core. The secondary<br />
structure tells about the possible alpha helices,<br />
beta sheets <strong>and</strong> coiled regions in the CtGH39.<br />
The Ramach<strong>and</strong>ran plot tells about the residues<br />
which have favourable conformation for psi <strong>and</strong><br />
phi angles which will further help in underst<strong>and</strong>ing<br />
the mechanism <strong>of</strong> cellulolysis.<br />
Materials <strong>and</strong> Methods<br />
The amino acid sequence <strong>of</strong> CtGH39<br />
(UniProt id A3DHB2) was first subjected to<br />
analysis by HHpred with 8 iterations <strong>of</strong> PSI-<br />
BLAST (16) for homology, it could also build a<br />
phylogenetic tree based on alignment scores so<br />
that evolutionary relationship among closely<br />
matching sequences can be understood. Thereafter,<br />
very closely resembling sequences with<br />
better E score