April Journal-2009.p65 - Association of Biotechnology and Pharmacy

Current Trends in Biotechnology and Pharmacy
Vol. 3 (2) 172-180, April 2009. ISSN 0973-8916
substrates did not cause enzyme productions as
high as wheat bran. Therefore, wheat bran has
been superior to other solid substrates for the
synthesis of á-amylase from Humicola
lanuginosa by solid state fermentation. The
maximum productivity of á-amylase (356 U/g)
was achieved by utilizing wheat bran as the solid
substrate with soluble starch as an additional
carbon source in 144 h at temperature 50 0 C and
moisture content of 90%, pH of 6, and inoculum
level of 20 % (w/v). Although the results of these
investigations are based on experiments
conducted in flasks, they provide valuable
information for the production of á-amylase by
solid state fermentation process at a larger scale.
Acknowledgement
We are very grateful to Dr. A.
Subrahmanyam, Head, Department of
Biotechnology & Microbiology, MIET, Meerut for
providing me a microbial strain.
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