April Journal-2009.p65 - Association of Biotechnology and Pharmacy

Current Trends in Biotechnology and Pharmacy
Vol. 3 (2) 155-161, April 2009. ISSN 0973-8916
Comparative UV-spectra of fermented cultural extract of
antifungal-active Streptomyces isolates recovered from different
ecological habitats
1
Ismail Saadoun, 1 Fouad AL-Momani, 1 Qotaiba Ababneh and 2 Shahidi Bonjar
1
Dept. of Biological Sciences, Jordan Univ. of Science and Technology, Irbid-22110, Jordan
2
Department of Plant Pathology, College of Agricultural Sciences, Bahonar University of Kerman, Kerman-Iran
* For Correspondence : isaadoun@just.edu.jo
Abstract
UV-spectra of antifungal-active
Streptomyces isolates were compared to
previously reported spectra and analyzed under
different extraction conditions. MU123
Streptomyces isolate from Turkey exhibited a UV
spectrum similar to 23 7
isolate from Jordan with 2
maximum absorbance peaks at (226 and 260 nm)
and at (220 and 260 nm), respectively. This
spectrum was repeated by the C5P1-6 isolate from
Jordan with 2 maximum absorbance peaks at 225-
250 and 300 nm. The aquatic species identified
as S. violaceusniger showed 2 maximum
absorbance peaks at 231 and 258 nm similar to
the UV spectrum of a clinical isolate of
actinomycetes (Streptomyces sp. 96.0333) that
exhibited 2 absorbance peaks at 220-225 and 262
nm. When C1P2-6 isolate from Jordan was
compared to Ir 102 from Iran under same cultural,
extraction and UV analysis conditions, data
revealed similar UV spectra with 2 absorbance
peaks for both isolates at 200-225 and 275-300
nm. Approximately 50% of reported active
screened isolates exhibit similar UV-spectra which
might reflect their habitats, culture and UV
analysis conditions. Comparison of UV-spectra
and absorption peaks of known antibiotics to that
of active Streptomyces isolates might explain the
ability of the same Streptomyces sp. to produce
several antibiotics.
Introduction
Members of the order Actinomycetales
are the most abundant soil microorganisms under
a wide variety of conditions. They include many
species that are characterized by the production
of extracellular important bioactive compounds.
Majority of those strains belong to species within
the genus Streptomyces which produce two–
thirds of the naturally occurring antibiotics world
wide. Such strains were advocated as promising
agents against several pathogens and are well
known for their potential to produce a large
number of inhibitory metabolites used in industry,
pharmacy, including: antihelminthic, antitumor,
antifungal agents (3, 4, 23). Several studies on
the isolation, characterization and genotyping of
soil streptomycetes of Jordan have already been
conducted (11, 13, 14, 15). Other studies showed
the ability of different streptomycetes isolates to
inhibit the growth of several multi-resistant Grampositive
and Gram-negative pathogens (18, 19,
20). Experiments on the nature of the inhibitory
metabolite produced by S. violaceusniger
showed a maximum absorption in the UV region
at 210-260 nm (16). In another study,
Streptomyces isolates active against Candida
albicans determined with UV-spectra
absorbance peaks were either at 230 nm or 300
nm or in between (17). Some of these spectra
were similar to the UV-spectra of the active
Comparison of UV spectrum analysis of active streptomycetes