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April Journal-2009.p65 - Association of Biotechnology and Pharmacy

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Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong><br />

Vol. 3 (2) 181-187, <strong>April</strong> 2009. ISSN 0973-8916<br />

186<br />

the major genetic determinant <strong>of</strong><br />

polymorphic activity in humans.<br />

Pharmacogenetics, 10: 415-424.<br />

Fig. 1: Electrophoretogram <strong>of</strong> Dde I digested<br />

PCR product generated by amplification <strong>of</strong><br />

genomic DNA using CYP3A5*3 gene<br />

specific primers.<br />

Lane # 1: 100 bp DNA ladder<br />

lane # 2: 3/3Genotype<br />

lane # 3,: 1/3Genotype<br />

lane # 4: 1/1Genotype<br />

Fig. 2: Electrophoretogram <strong>of</strong> Dde I digested<br />

PCR product generated by amplification <strong>of</strong><br />

genomic DNA using CYP3A5*6 gene<br />

specific primers.<br />

Lane # 1-4: 1/1<br />

lane # 5: Uncut b<strong>and</strong><br />

lane # 6,: 100 bp DNA ladder<br />

Reference<br />

1. Paulussen A., Lavrijsen K., Bohets H.,<br />

Hendrickx J., Verhasselt P., Luyten W.,<br />

Konings F. <strong>and</strong> Armstrong M. (2000). Two<br />

linked mutations in transcriptional regulatory<br />

elements <strong>of</strong> the CYP3A5 gene constitute<br />

2. Gellner K., Eiselt R., Hustert E., Arnold H.,<br />

Koch I., Haberl M., Deglmann CJ., Burk<br />

O., Buntefuss D., Escher S., Bishop C.,<br />

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Kleine K., Meyer UA. <strong>and</strong> Wojnowski L.<br />

(2001). Genomic organization <strong>of</strong> the human<br />

CYP3A locus: identification <strong>of</strong> a new,<br />

inducible CYP3A gene. Pharmacogenetics,<br />

11: 111-121.<br />

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Vilarem MJ. (1994). Sequence <strong>of</strong> the 5-<br />

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<strong>and</strong> Schuetz E. (2001). Sequence diversity<br />

in CYP3A promoters <strong>and</strong> characterization<br />

<strong>of</strong> the genetic basis <strong>of</strong> polymorphic<br />

CYP3A5 expression. Nature Gene, 27: 383-<br />

391.<br />

5. Park SY., Kang YS., Jeong MS., Yoon<br />

HK. <strong>and</strong> Han KO. (2008). Frequencies<br />

<strong>of</strong> CYP3A5 genotypes <strong>and</strong> haplotypes in<br />

a Korean population. Pharmacogenetics,<br />

33 (1): 61-65.<br />

6. Liu TC., Lin SF., Chen TP. <strong>and</strong> Chang JG.<br />

(2001). Polymorphism analysis <strong>of</strong> CYP3A5<br />

in myeloid leukemia. Oncol Rep,9(2):327-<br />

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7. Nuremberg JI. <strong>and</strong> Lahari D KJr. (1991).<br />

A rapid non-enzymatic method for the<br />

Surekha et al

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