April Journal-2009.p65 - Association of Biotechnology and Pharmacy
April Journal-2009.p65 - Association of Biotechnology and Pharmacy
April Journal-2009.p65 - Association of Biotechnology and Pharmacy
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
Current Trends in <strong>Biotechnology</strong> <strong>and</strong> <strong>Pharmacy</strong><br />
Vol. 3 (2) 172-180, <strong>April</strong> 2009. ISSN 0973-8916<br />
imposed by the solid nature <strong>of</strong> the substrate <strong>and</strong><br />
leads to a decrease in growth <strong>and</strong> enzyme<br />
production (5). Lower inoculum level results in a<br />
lower number <strong>of</strong> cells in the production medium.<br />
This requires a longer time to grow to an optimum<br />
number to utilize the substrate <strong>and</strong> to form the<br />
desired product (40). In the present study, the<br />
maximum amylase activity was found at 20 % <strong>of</strong><br />
inoculum level. After this inoculum concentration<br />
no significant increase in enzyme activity has been<br />
found (Fig.5). This may be due to the limiting<br />
nutrients at higher inoculum size.<br />
177<br />
incubation time <strong>and</strong> after 144 h the enzyme<br />
production decreases due to substrate inhibition.<br />
Thus, the maximum enzyme has been produced<br />
at 144 h <strong>of</strong> incubation time. A similar result has<br />
been reported by Ellaiah et al. (37).<br />
Effect <strong>of</strong> supplementation <strong>of</strong> carbon sources<br />
on á-amylase activity<br />
The influence <strong>of</strong> four supplementary<br />
carbon sources has been studied. These carbon<br />
sources are soluble starch, sucrose, maltose,<br />
glucose. Among all supplementary carbon sources,<br />
the soluble starch has been found to be the best<br />
source for maximum amylase production (Fig.7).<br />
Fig. 5. Effect <strong>of</strong> inoculum size on á-amylase activity<br />
Fig. 7. Effect <strong>of</strong> supplementary carbon sources on á-<br />
amylase activity<br />
Fig. 6. Effect <strong>of</strong> incubation time on α-amylase activity<br />
Effect <strong>of</strong> incubation time on á-amylase<br />
activity<br />
Figure.6 shows the variation in amylase<br />
activity with incubation time at temperature 50<br />
0<br />
C <strong>and</strong> pH 6.The trend indicates that the amylase<br />
production increases with the increase in<br />
The data corresponding to control in figure-7<br />
indicates the production without additional carbon<br />
source. In previous studies, the soluble starch was<br />
also reported as the best carbon supplement for<br />
amylase production in M. thermophila D14 (8)<br />
<strong>and</strong> A. fumigatus (41,42).<br />
Conclusion<br />
The use <strong>of</strong> solid state fermentation (SSF)<br />
for production <strong>of</strong> á-amylase using Humicola<br />
lanuginosa is an economical process <strong>and</strong> is very<br />
simple to apply. All the solid substrates wheat bran,<br />
corncob leaf, wheat straw, rye straw can be used<br />
for supported biosynthesis <strong>of</strong> α-amylase using H.<br />
lanuginosa under SSF. However, these<br />
Ravi et al