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1350 Ump101 INDUCED EXPRESSION OF PIRES-EGR1-EGFP-OMI/ HTRA2 AND ITS EFFECT ON APOPTOSIS OF HUMAN CHOROIDAL MELANOMA OCM-1 CELLS Xufang Sun, Tian Yu, Shu Yan (applesxf@yahoo.com.cn) Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology; Wuhan Aier Eye Hospital, Wuhan, China Purpose. The hydrogen peroxide induced and the kill characteristics of pIRES-Egr1-EGFP-Omi/HtrA2 in human choroidal melanoma OCM-1 cells were investigated. Methods. pIRES-Egr1-EGFP and pIRES-Egr1-EGFP-Omi/HtrA2 recombinant plasmids were constructed and RT-PCR assays were used to identify, which were transfected into the cultured OCM-1 cells by lipofectamineTM2000. The cells transfected by both plasmids were stimulated respectively by hydrogen peroxide. pIRES-Egr1-EGFP transfected cells were used as control groups. The expression levels of Omi/HtrA2 and XIAP were examined by Western blotting. The rations of apoptotic cells were assessed by flow cytometry. The transfection rates was observed by fluorescence microscopy. MTT method was used for observing cell proliferation. Results. Our Results showed that the recombinant plasmids could successfully constructed and transferred into OCM-1 cells by lipofectamineTM2000. Compared with the control group, the Omi/ HtrA2 and XIAP gene expression at the protein level were decreased 53.5% and 31.9%, respectively. The apoptotic ratio of pIRES-Egr1-EGFP- Omi/HtrA2 transferred cells increased significantly after the induction (p
1949 Ump104 GENDER DIFFERENCES AND ESTROGEN AND PRO- GESTERONE RECEPTOR EXPRESSION IN UVEAL MEL- ANOMA L. Schoenfield, M. Turell, P. Carver, S. Mackie, R. Tubbs, A. Singh (schoenl@ccf.org) Cleveland Clinic, Cleveland, OH; Pathology and Lab Medicine Institute; Cole Eye Institute Purpose. Older reports have not demonstrated estrogen receptor (ER) expression in uveal melanoma (UVM). A recent study reporting differences in incidence and metastasis-related mortality by gender prompted a re-examination of ER and progesterone receptor (PR) expression by immunohistochemistry with comparison to clinical outcome and chromosome 3 status. Methods. 33 cases of UVM from 2004-09 treated by enucleation were examined for incidence and survival. 23 cases were evaluated for ER and PR by immunohistochemistry. Chromosome 3 status was determined by FISH and SNP array analysis. Results. There were 19 men (58%) and 14 women (42%). 11 patients were DOD: 8 men (67%) and 3 women (25%); 1 woman was alive with metastasis. 10 men (56%) and 8 women (44%) were alive without metastasis. 3 patients died of other causes. ER was positive in 7 women (11 positive cases) and negative in UVM arising in men (8 of 12 negative cases). PR was negative in 20 of 23 cases and weakly positive or negative in 3. ER did not predict either clinical outcome or chromosome 3 status. Of the ER positive cases, 4 were DOD and 6 were alive without metastasis. Of the ER negative cases, 5 were DOD and 6 alive without metastasis. When compared to chromosome 3 abnormalities, ER was positive in 9/18 monosomy 3 cases and 2/5 disomy cases. Conclusions. Gender may play a role in UVM. ER expression was present in 48% of cases of uveal melanoma and more likely in women in this small study, but it does not appear to be prognostic. We propose further study, including quantitative evaluation using image analysis. Financial disclosure. None 2320 Ump105 DOES INTERNAL BLOOD FLOW VELOCITY CORRELATE WITH KNOWN PROGNOSTIC FACTORS FOR PRIMARY UVEAL TRACT MELANOMA? Amit Arora, Vasilios P. Papastefanou, Mandeep S Sagoo, Marie Restori, Marianne Graham, Victoria ML Cohen (amitkarora@hotmail.com) Ocular Oncology Service, St Bartholomew’s Hospital & Moorfields Eye Hospital, London Institute of Ophthalmology, University College London Cytogenetics Department, Barts and the London NHS Trust Purpose. To examine the association of internal blood flow velocity in primary uveal melanoma with tumour size, chromosomes 3/8 status and histopathology type. Methods. Between 2009 and 2011, all patients with large uveal tract melanoma scheduled for enucleation underwent internal blood flow velocity studies using B-mode Doppler ultrasound scans at the time of diagnosis. Tumour height and diameters were clinically determined. Corresponding areas and volumes were calculated for each tumour. UVEAL MELANOMA Posters 109 Punch biopsy tissue samples were obtained at the time of surgery for cytogenetic analysis for the status of chromosomes 3 and 8 with interphase FISH analysis. Cell type was determined by histopathology. Spearman correlation coefficient was used to examine the association of internal blood flow velocity with tumour dimensions. Results. Twenty-three enucleation specimens underwent preoperative blood flow velocity studies. Internal blood flow was not positively correlated with tumour height (r=0.061, p=0.786), area (r=-0.1.7, p=0.636) or volume (r=0.015, p=0.946). Similarly, no significant difference was noted in regards to cell type (12.25 ± 4.99 cm/sec for epithelioid cells, 16.1± 10.22 cm/sec for mixed cell and 11.7± 6.9 cm/sec for spindle cell tumours respectively [mean ± 1SD] or chromosome 3/8 status. Conclusions. Internal blood flow of primary uveal melanoma does not appear to be associated with uveal melanoma cell type or chromosome status. No positive correlation was found in relation to tumour dimensions. Financial disclosure. None 1425 Ump106 EVALUATION OF IRIS AND CILIARY BODY LESIONS WITH ANTERIOR SEGMENT OPTICAL COHERENCE TOMOGRAPHY (AS-OCT) VERSUS ULTRASOUND B SCAN M. S. Sagoo1,2,3, V. P. Papastefanou2,3, S. Hau2, S. Shah2, M. Tsimpida2,3, V.M. Cohen2,3 (mandeep.sagoo@moorfields.nhs.uk) 1. UCL Institute of Ophthalmology 2. Moorfields Eye Hospital, London 3. Ocular Oncology Service, Barts and the London NHS Trust, London Purpose. To compare anterior segment optical coherence tomography (AS- OCT) versus ultrasound B scan for imaging iris and ciliary body lesions. Methods. We prospectively evaluated 66 eyes with AS-OCT and ultrasound B scan. Comparison of images between the two imaging modalities was made. Results. Thirty seven eyes (56%) had iris naevus, 13 (20%) iris pigment epithelial cyst, 6 (9%) iris melanoma, 2 (3%) iris haemangioma, 7 (10%) other iris lesions, and 1 (2%) ciliary body melanoma. Image analysis revealed (AS-OCT vs ultrasound B scan) the ability to detect lesion (62/66 (94%) vs 46/66 (70%), complete visualisation of the lesion in 26/62 (42%) vs 46/46 (100%), and posterior lesion shadowing in 51/62 (82%) vs 0/46 (0%). Conclusions. AS-OCT offers better detection and precise measurement but is less able to provide complete visualisation of iris lesions compared with ultrasound. Financial disclosure. None 114 Ump107 Β-RADIATION BRACHYTHERAPY FOR LARGE CHOROIDAL MELANOMA: DO WE NEED A HIGH DOSE TO THE APEX? M. Naseripour, K.H. Ghasemi Falavarjani, R. Jaberi, A.R. Irani, Z. Azma (masoodnp@yahoo.com) Eye Research Center, Rassoul Akram Hospital, Tehran University of Medical Sciences
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XV th NH City Hotel and Tower Boliv
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List of Congress Meetings of the In
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Table of Contents Welcome address K
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Keynote Lectures Lymphoma of the oc
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The venue for the Biannual Meeting
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Buenos Aires Downtown MAP 12 Venue
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MORNING 8.30-10.10 Papers (Moderato
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50 RES 26 USING THE GLYCOLYTIC INHI
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2226 RES 4 NOTCH SIGNALING PROMOTES
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a reduction in the number of living
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array further detected copy number
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615 RES 21 TRB2 AND SKP2 IN THE RET
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following treatment with 2-fluorode
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1909 Rb14 RESULTS OF PATIENTS WITH
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Posters Retinoblastoma 2006 RBp100
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120 RBp135 MANAGEMENT OF AN ECTOPIC
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After treatment, 10 patients lived
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Methods. Six cycles of carboplatin
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1940 RB15 PROTON THERAPY FOR RECURR
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4. Fluoroscopy for cannula placemen
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Methods. A retrospective chart revi
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Ruth A. Kleinerman1, Chu-ling Yu1,
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2006 RBp100 RETINOBLASTOMA ASSESSME
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months. Mean delay between beginnin
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not associated with severity of RB.
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Conclusions. During a ten-year peri
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30 RBp127 TREATMENT MODULATION IN R
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Page 116: Methods. A total of 4070 patients w
Page 119: First authors Naseripour, Masood No
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