Tissues to be placed in 10 % formalin. Please save 2 sets of tissues.qqqqqqqqqqqqqqqqqqqqqqqqqqSkin: full thickness of abdominal skin.Skeletal muscle: medial thigh, with sciatic nerve.Tongue: Cross section near tip including both mucosal surfaces.TracheaThyroid/parathyroidThymus: representative section.Lungs: section from several lobes including a major bronchus.Heart: Longitudinal section including atrium, ventricle and valves form both right and left heart.Include large vessels.AortaSalivary glandGastrointestinal tract: 2-3 cm long section of esophagus, stomach (cardia, antrum, pylorus),duodenum, jejunum, ileum, colon, omentum. Open carefully along the long axis.Lymph nodes: Cervical, bronchial, and mesenteric with a transverse cut.Liver: Sections from several lobes with capsule and gall bladder.Adrenal: Incise transversely.Female reproductive tract: Entire uterus and ovaries with longitudinal cut into lumen. Entire testiswith transverse cut, entire prostate with transverse cut. This should be sent to Dr. Linda Munson.Pancreas: Representative sections from 2 areas.Spleen: Cross sections including capsule.Kidneys: Section from both kidneys (cortex, medulla, and pelvis).Urinary bladder/ureter/urethra: Cross section of bladder, 2-cm sections of ureters, cross section ofurethra.Eyes: leave intact.Brain, include cerebrum and cerebellum: Sliced longitudinally along the midline.Pituitary gland: Bisect gland including dura, if possible. Otherwise submit entire gland to localpathologist.Long bone: Submit ½ of femur.Mammary glandDiaphragmSpinal cord: 1-cm section from cervical cord.<strong>Asian</strong> <strong>Small</strong>-<strong>Clawed</strong> <strong>Otter</strong> <strong>Husbandry</strong> <strong>Manual</strong>/Health Care-30-
Diseases of the <strong>Asian</strong> small-clawed otterUroliths and dental problems were the most commonly reported diseases of the <strong>Asian</strong> small-clawedotter in a survey conducted by the SSP in 1997-<strong>1998</strong>. Other problems frequently encounteredincluded abscesses, (usually due to intraspecies aggression), lameness (often self-limiting), andoccasional vomiting and diarrhea. A couple of institutions reported fur pulling, which appeared to berelated to the environmental stress of overcrowding. Non-specific dermatitis was also reported fromtwo institutions. Other health problems listed infrequently in the survey included spinal disease,intussusception, pyothorax, salmonellosis, corneal ulcer, ruptured cruciate ligament, and diabetes.Urolithiasis:Urolithiasis is a prevalent disease in the captive population of <strong>Asian</strong> small clawed otters with theincidence being as high as 66 % (Calle, 1988). The most common component of uroliths in this speciesis calcium oxalate, however urate calculi have also been reported. Most of these calculi are sterilehowever there are exceptions. Calculi are most frequently located in the kidneys; however, someanimals develop cystic calculi as well. Rarely does an otter have cystic calculi without also havingkidney stones. Many small-clawed otters will develop calculi by 2 years of age, and most are affectedby 4 or 5 years Both males and females are affected. The calculi generally do not cause clinical signs ofdisease in the early stages and radiographs are required to diagnose the condition. <strong>Small</strong> renal calculican usually be seen on radiographs before they are visible by ultrasound. In most cases thecalculi get progressively larger as the animal matures. Larger renal calculi diminish urine flow andhistopathologic changes occur in the renal parenchyma, getting more severe with age. Older animalswith large renaliths may have elevated serum BUN and creatinine serum levels. Cystic calculi maycause hematuria and dysuria, but many animals with cystic calculi remain asymptomatic. Rarely do thecalculi cause a complete blockage of the urinary tract.Treatment:No successful treatment for this disease has yet been identified. Lithotripsy has been attempted in onecase with little success. Cystic calculi can be successfully removed by surgery, but often reoccur. Afew veterinarians have removed some larger renaliths surgically from the renal pelvis. This maytemporarily alleviate some of the symptoms, although, as with cystic calculi, the stones often reoccur.Recommendations:Radiograph all small-clawed otters beginning at six month of age to check for calculi. After that,annual examinations and radiographs should be performed. The number and size of the calculi presentin the left kidney, the right kidney, and the urinary bladder should be recorded.All otters with urolithiasis should have a complete medical work-up performed including CBC,chemistries, urinalysis, and an aerobic and anaerobic culture of the urine. Bacterial infections should betreated according to the results of cultures and sensitivities. Follow-up cultures should be performedperiodically to assure infections have been eliminated. Cystic calculi should be removed surgically to<strong>Asian</strong> <strong>Small</strong>-<strong>Clawed</strong> <strong>Otter</strong> <strong>Husbandry</strong> <strong>Manual</strong>/Health Care-31-