products - Integrated DNA Technologies

INTEGRATEDDNATECHNOLOGIESPRODUCTSFUNCTIONAL GENOMICSExogenous Reporter Gene Positive ControlsReporter genes can be used both as positive controls and as negative controls and so are very useful reagents. If your cell lineexpresses the reporter either stably or via co-transfection of an expression plasmid, the anti-EGFP or anti-FLuc DsiRNAs canfunction as a positive control. If your cell line does not express these reporter genes, then the anti-EGFP or anti-Luciferase(Firely or Renilla) DsiRNAs can function as negative controls. Importantly, these DsiRNAs are validated, functional duplexeswith known efficient RISC loading and therefore offer an added level of control that non-targeting sequences cannot provide.ProductEGFP-S1 DS Positive Control - 1 nmole or 5 nmolesFLuc-S1 DS Positive Control (pGL2, pGL3) - 1 nmole or 5 nmolesFLuc-S2 DS Positive Control (pGL4) - 1 nmole or 5 nmoleshRLuc-4 DS Positive Control - 1 nmole or 5 nmolesUniversal Negative ControlA negative control duplex has been developed that does not target any sequence in the human, mouse, or rat transcriptomes.It can be employed as a universal negative control for DsiRNA transfections. This is a non-targeting sequence. The EGFP andFLuc DsiRNAs may also be used as negative controls if functional, targeting duplexes are desired.ProductDS Scrambled Neg - 1 nmole or 5 nmoles©2008 Integrated DNA Technologies 22 www.idtdna.com