products - Integrated DNA Technologies

INTEGRATEDDNATECHNOLOGIESPRODUCTSFLUORESCENCE-BASED APPLICATIONSRNase SubstratesRNase Substrates are a collection of four fluorescent dual-labeled oligonucleotide probes that can be used to quantitatively orqualitatively detect RNase activity with any base specificity. Intact probes are dark (quenched) while cleaved probes are bright(unquenched). Assays are rapid, quantitative over time, inexpensive, and do not involve the use of radioactive or otherwisehazardous reagents. Each substrate contains only a single ribonucleotide residue: rU, rC, rA or rG 1-2 .Product Sequence QuantityrU RNase Substrate 5’ 6-FAM-ArUAA-3’ TAMRA 10 nmolesrC RNase Substrate 5’ 6-FAM-ArCAA-3’ TAMRA 10 nmolesrA RNase Substrate 5’ 6-FAM-ArAAA-3’ TAMRA 10 nmolesrG RNase Substrate 5’ 6-FAM-ArGAA-3’ TAMRA 10 nmolesRNase Substrates were developed in collaboration with Professor Ron Raines from the University of Wisconsin, Madison.References:1. Hypersensitive substrate for ribonucleases. Kelemen, B.R., Klink, T.A., Behlke, M.A., Eubanks, S.R., Leland, P.A., and Raines, R.T. Nucleic Acids Res. 27:3696-3701 (1999).2. Fast, facile, hypersensitive assays for ribonucleolytic activity. Park, C., Kelemen, B.R., Klink, T.A., Sweeney, R.Y., Behlke, M.A., Eubanks, S.R., and Raines, R.T.Methods Enzymol. 341:81-94 (2001).©2008 Integrated DNA Technologies 58 www.idtdna.com