products - Integrated DNA Technologies

INTEGRATEDDNATECHNOLOGIESPRODUCTSMODIFICATIONSKeyAll modifications listed are available on oligos with DNAbases. The following symbols indicate additional options.Freedom Dye equivalent availableAvailable on oligos with RNA basesTechnical NoteA wide variety of modifications can be incorporated into an oligonucleotide at the time of synthesis. When possible,this is done using a modified solid support (CPG) for 3’-modifications or a specialized phosphoramidite reagent forinternal and 5’-modifications.Certain modifications (notably Digoxigenin and some fluorescent dyes) are not available as a modified-CPG orphosphoramidite and must be attached to the oligo after synthesis using NHS Ester chemistry. NHS Esters react withfree primary amines and result in stable, covalent attachment. A primary amine is therefore added to the oligo duringsynthesis to permit reaction with the desired NHS Ester.5’ 5’ Amino Modifier C6 (phosphoramidite)Int Internal amino-C6-dT (phosphoramidite)3’ 3’ Amino Modifier (CPG)Post-synthetic chemical modifications made to an oligonucleotide result in lower yields than modifications introducedduring synthesis. Further, all NHS Ester modifications require HPLC purification. PAGE purification is not offered forNHS Ester modifications as yields are further decreased and certain modifications can be damaged during PAGEpurification.Listed molecular weights correspond to the increase in MW of the oligo upon addition of the modification andincludes attachment groups used to incorporate the modification into the sequence. In some cases, the linker, andhence the MW, differs for attachment to the 5’ end, internal sites or 3’ end.Absorbance and Emission maxima of fluorescent dyes may vary slightly with oligo composition. Listed Ab/Em Maxwere measured on a standardized oligo sequence in 10 mM Tris, 50 mM KCl, 5 mM MgCl2, pH 8.3.All modifications are not available on every synthesis scale. Please visit www.idtdna.com or contact a local sales officefor more information.Please inquire for modifications not listed.©2008 Integrated DNA Technologies 60 www.idtdna.com