products - Integrated DNA Technologies

INTEGRATEDDNATECHNOLOGIESPRODUCTSMODIFICATIONSAttachment Chemistry / LinkersThiol ModificationsA thiol group can be used to attach an oligo to a variety of fluorescent and nonfluorescent moieties or surfaces. Oligoscontaining thiol modifiers are shipped in their oxidized (disulfide) form and require chemical reduction by dithiothreitol (DTT)or Tris (2-carboxyethyl) phosphine (TCEP) prior to use.Thiol Modifier C3 S-S MW: 244.35’ INT. 3’— — a'5OSSOHHOSThiol Modifier C6 S-S MW: 328.4S5’ INT. 3’a — —OO P O -O3'Dark QuenchersIowa Black® QuenchersThe Iowa Black quenchers are a family of proprietary dark quenchers developed by IDT that are offered as either 5’- or 3’-endmodifications. They have excellent properties for use in fluorescence-quenched probes. These compounds are stable over awide range of conditions, including pH and heat.Iowa Black® FQ Ab max531 nm 5’ MW: 426.43’ MW: 583.6Iowa Black FQ has a broad absorbance spectra ranging from 420 to 620 nm with peak absorbance at 531 nm. This quencher isideal for use with fluorescein and other fluorescent dyes that emit in the green to pink spectral range.5’ INT. 3’a — aHPLC purification requiredIowa Black® RQ 5’ Ab max656 nm 5’ MW: 420.43’ Ab max667 nm 3’ MW: 678.6Iowa Black RQ has a broad absorbance spectra ranging from 500 to 700 nm. This quencher is ideal for use with TEX 615, TYE563, TYE 665, and other fluorescent dyes that emit in the red spectral range.5’ INT. 3’a — aHPLC purification required©2008 Integrated DNA Technologies www.idtdna.com73