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INTEGRATEDDNATECHNOLOGIESAppendixPRODUCTSAPPENDIXStandard IDT Ordering Base Codes• Standard Bases: A, C, G, T• RNA expressed as ‘r_’ (for example rA, rU)• 2’ O-Methyl RNA bases are entered as ‘m_’.• Locked nucleic acid bases (LNA) are entered as ‘+_’.• Phosphorothioated DNA bases are entered as ‘_*’• Phosphorothioated RNA bases are entered as ‘r_*’.• Phosphorothioated 2’-O-Methyl RNA bases are entered as ‘m_*’.• Phosphorothioated LNA bases are entered as ‘+_*’.Please note that rT and mT are not valid, and must be expressed as rU and mU instead.Mixed Base DefinitionsMixed bases, which are also known as degenerate or wobble bases, can be introduced at any position in an oligomer sequence. Forexample, mixed-base composition at a single position can include all 4 bases (“N”), C or T bases (“Y”), A or G bases (“R”), etc. Since there are11 different possible combinations of 2, 3 or 4 bases, a universal nomenclature has been established that must be used when specifyingnucleic acid content at a mixed-base site.IUB Codes:SymbolRYMKSWHBVDNMixed BasesA, GC, TA, CG, TC, GA, TA, C, TC, G, TA, C, GA, G, TA, C, G, TModification ListCategory Modification Sequence Code Molecular Weight Ab maxEm maxAttachment Chemistry/Linkers 3’ Amino Modifier /3AmM/ 179.23’ Biotin /3Bio/ 437.53’ Biotin-TEG /3BioTEG/ 569.63’ Cholesteryl-TEG /3CholTEG/ 756.03’ Digoxigenin NHS Ester /3DigoxN/ 722.93’ Thiol Modifier C3 S-S (Disulfide) /3ThioMC3-D/ 244.35’ Acrydite /5Acryd/ 247.25’ Amino Modifier C12 /5AmMC12/ 263.35’ Amino Modifier C6 /5AmMC6/ 179.25’ Amino Modifier C6 dT /5AmMC6T/ 458.45’ Biotin /5Bio/ 405.45’ Biotin dT /5BiodT/ 684.75’ Biotin-TEG /5BioTEG/ 569.65’ Digoxigenin NHS Ester /5DigN/ 722.9©2008 Integrated DNA Technologies 86 www.idtdna.com