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INTEGRATEDDNATECHNOLOGIESPRODUCTS©2008 Integrated DNA Technologies www.idtdna.com33
INTEGRATEDDNATECHNOLOGIESMICROARRAY PRODUCTSMicroarray ProductsMicroarrays have become a routine tool used in many labs for applications ranging from quantitative analysis of geneexpression profiles to SNP genotyping.Microarray BioinformaticsCorrect design of oligonucleotides used in microarray experiments requires that a variety of parameters be considered. Oligosmust be screened for Tm, self-dimer potential, hairpin potential, location within the transcript, and cross-reactivity with othertranscripts.IDT Bioinformatics offers a free microarray oligo design tool on the web in the Workbench section of SciTools (www.idtdna.com/scitools/scitools.aspx). This design tool allows for batch processing of thousands of probes or single oligos.SpotQCIDT has developed a Cy3 TM dye-labeled oligonucleotide with improved thermodynamics that will hybridize with greateruniformity to the wide variety of sequences employed in large-scale microarrays. Our Cy3 TM detector oligo used with ouroptimized hybridization solution permits rapid, precise QC of printed microarray slides. Hybridization, wash, and imageacquisition can be done in under an hour. While the benefit of our new formulation is most evident with oligonucleotidearrays, SpotQC can also be used with superior results for cDNA/PCR product arrays. SpotQC has improved sensitivity, detectsa greater range of oligo-probe lengths and GC compositions, and more accurately assesses the spotted probe’s availability forhybridization vs. traditional random fluorescent primers. Biotin SpotQC employs a biotin-labeled detector oligo that permitsthe use of enzymatic-based chemiluminescent or colorimetric detection options for the QC assay.Cy3 SpotQC Detector OligoProduct1000 pmoles Cy3 SpotQC Detector OligoBiotin SpotQC Detector oligoBio-SpotQC is a biotinylated version of SpotQC, and has been developed for precise QC of printed microarray slidesutilizing secondary fluorescent, chemiluminescent, or colorimetric hybridization detection assays. (Secondary fluorescent,chemiluminescent, and/or colorimetric detection assay reagents not included.)Product1000 pmoles Bio-SpotQC Detector OligoDetector oligos are shipped with a complimentary tube of 1X Hybridization Buffer (500 µl). The quantity is sufficient to analyze10 arrays using large 24x60mm cover slips or up to 30 arrays using small 22x22mm cover slips.©2008 Integrated DNA Technologies 34 www.idtdna.com
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