products - Integrated DNA Technologies

INTEGRATEDDNATECHNOLOGIESPRODUCTSMODIFICATIONSAttachment Chemistry / Linkers3’ Cholesteryl TEG MW: 756.0Cholesterol can be conjugated to oligonucleotides and can facilitate uptake into cells. It has been used as a transfection aidfor antisense oligos and siRNAs, both in vitro and in vivo. Cholesterol is a very hydrophobic modification which is best purifiedusing RP-HPLC.5’ INT. 3’— — aOOO5'OHPLC purification requiredONHOOOHDigoxigenin NHS Ester MW: 722.9Digoxigenin is a small hapten that can be conjugated to amino-modified oligos. Anti-digoxigenin antibodies allow capture ordetection of a digoxigenin-labeled oligo and can be used in a variety of assay formats much like biotin/streptavidin.5’ INT. 3’a — aOHCH3OOHPLC purification requiredOCH3HNOHNHODithiol MW: 214.2Each Dithiol insertion results in 2 SH groups and is available for coupling with ligands or surfaces (linkage to gold surfacesin one popular application). Dithiol can be inserted in series so that two or even three groups can be positioned adjacentto each other to increase efficiency of ligand/surface interactions. Note that serial insertions will decrease oligo yield due tolower efficiency of incorporation of the Dithiol phosphoramidite.O5'5’ INT. 3’a a —SSOOO P O -Additional purification requiredO3'I-Linker MW: 208.2I-Linker, developed at IDT, is a proprietary covalent attachment chemistry for oligonucleotides. The modifier is attached to the5’-end of the oligo. I-Linker can be substituted for amino modifications in many applications. In addition, I-Linker expands therange of reactive groups that can be used for conjugation, including aldehyde and ketone-modified ligands or surfaces.5’ INT. 3’a — —©2008 Integrated DNA Technologies 72 www.idtdna.com