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INTEGRATEDDNATECHNOLOGIESPRODUCTSNUCLEASE CONTROLRNaseAlert and DNaseAlert SubstrateNuclease Detection SystemsNucleases are widely present in the laboratory environment and can interfere with many experiments. Single-strandedRNases are ubiquitous, hard to eliminate and can rapidly degrade important samples used in microarray studies, real-timePCR, Northern blots or cDNA cloning. IDT has developed reagents that allow for rapid, sensitive detection of RNases andDNases. These reagents are fluorescence-quenched oligonucleotide probes that emit a fluorescent signal only after nucleasedegradation. Assays can be used qualitatively to test lab reagents, equipment and supplies for nuclease contamination.Assays can also be used quantitatively to study enzyme kinetics.The RNaseAlert substrate employs a FAM (fluorescein) reporter (Em 520 nm) and a dark quencher. The RNA sequence hasbeen carefully optimized to react with a wide variety of ribonucleases. Intact, the substrate has little or no fluorescence. Whencleaved by an RNase, the substrate fluoresces green (490 nm or UV excitation, 520 nm emission).The DNaseAlert Substrate is a synthetic DNA oligonucleotide that has a HEX reporter dye (hexachlorofluorescein) on oneend and a dark quencher on the other end. The DNA sequence has been carefully optimized to react with a wide variety ofnucleases; it contains domains that will react with single-stranded endonucleases, certain single-stranded exonucleases,and double-stranded nucleases. Intact, the substrate has little or no fluorescence. When cleaved by a DNase, the substratefluoresces pink (536 nm or UV excitation, 556 nm emission) and can be detected visually or using a fluorometer.RNaseAlert and DNaseAlert are available in single-use tubes that are ideal for rapid hand-held visual assays at the researchbench, or as bulk substrate that can be used in either visual assays or read in a fluorometer. Buffers are optimized for detectionof a wide variety of DNases or RNases.RNaseAlert Kit Components:• (25) 50 pmole Fluorescent Substrate tubes• 250 µl RNaseAlert Buffer• 2 mL Nuclease-Free Water• 50 µl RNase A (positive control)• 50 mL Nuclease Decontamination SolutionDNaseAlert Kit Components:• (25) 50 pmole Fluorescent Substrate tubes• 250 µl DNaseAlert Buffer• 2 mL Nuclease-Free Water• 25 µl DNase 1 Enzyme (positive control)• 50 mL Nuclease Decontamination SolutionKit quality is guaranteed for six months from the date received when stored at -20°C.Nuclease Detection Products(25) single-use 50 pmole tubes(2) 2-nmole bulk tubes (enough for 200 reactions)Complete Kit10X Buffer 2 mL tubeRelated ProductsRNase Substrates Pg. 58Nuclease Decontamination Solution Pg. 40©2008 Integrated DNA Technologies 38 www.idtdna.com