products - Integrated DNA Technologies

INTEGRATEDDNATECHNOLOGIESPRODUCTSMODIFICATIONSAttachment Chemistry / LinkersBiotin dT MW: 684.7HNONH5’ INT. 3’HNOONHHNSa a —'5OOONOHPLC purification requiredOO P O -Biotin-TEGBiotin-TEG has an extended 15-atom spacer arm.O3'MW: 569.6O5’ INT. 3’HNNHa — aSHNOOOOO5'OAdditional purification is required on 5’ Biotin-TEG.OO P O -O3'Dual Biotin MW: 871.0Two biotin groups are sequentially placed on the 5’-end, which increases the efficiency of streptavidin binding. Dual Biotin iscommonly employed in SAGE protocols 1 O.HNNH5’ INT. 3’HNOHa — —Additional purification requiredHNSONHOHNOOPOO -SReference:1. Enhanced concatemer cloning-a modification to the SAGE (Serial Analysis of Gene Expression) technique. Powell, J., Nucleic Acids Research, 26:3445-46(1998).OOO P O -O3'PC Biotin MW: 597.6PC Biotin produces a photocleavable spacer between the biotin group and the DNA bases. Optimal cleavage is obtained withexposure to long-wave UV light in the 300-350 nm spectral range. Cleavage releases the oligo with a 5’-phosphate group.5’ INT. 3’OHNNHa — — HNHPLC purification requiredSONHNO 2OOO P O -O3'©2008 Integrated DNA Technologies www.idtdna.com71