products - Integrated DNA Technologies

INTEGRATEDDNATECHNOLOGIESPRODUCTSMODIFICATIONSFluorophores6-FAM (Fluorescein) Ab max495 nm Em max520 nm 5’ MW: 537.53’ MW: 569.56-carboxyfluorescein is a single isomer derivative of fluorescein. 6-FAM is the most commonly used fluorescent dye forattachment to oligonucleotides and is compatible with most fluorescence detection equipment. Below pH 7, 6-FAMbecomes protonated, which results in decreased fluorescence. It is typically used in the pH range 7.5-8.5.5’ INT. 3’-OOOa — aOO –OHN5' 6-FAMOOFluorescein-dT Ab max495 nm Em max520 nm 3'MW: 816.7Fluorescein-dT is a modified base wherein fluorescein is attached to position 5 of the thymine ring by a 6-carbon spacer arm.O P O --OOO5’ INT. 3’O –a a —HNOONHHNOOHPLC purification required'5OOONOO P O -O3'HEX Ab max538 nm Em max555 nm MW: 744.1Hexachlorofluorescein is a chemical relative of fluorescein that is commonly used for multiplexed assays with FAM and TET.5’ INT. 3’-OClOClOa — —ClClO –ClOOClHNO P OJOE NHS Ester Ab max529 nm Em max555 nmOMW: 666.4- 3'JOE is 6-carboxy-4’, 5’-dichloro-2’, 7’- dimethoxyfluorescein. It is available only as an NHS ester and must be attached to anamino-modified oligonucleotide. This dye is frequently used in multiplex reactions with fluorescein.O5’ INT. 3’ClCla — a-OOOHPLC purification requiredH 3COO –OCH 3HNOO©2008 Integrated DNA Technologies 66 www.idtdna.com