2008 Summer Meeting - Leeds - The Pathological Society of Great ...

P99Cystic Monodermal Teratoma of Neurogenic TypeR Al Shamsi 1 , D Kermani 1 , B Kaur 1 , S El Sheikh 11 Royal Free Hospital, LondonWe report a case of a 28 years old lady, found to have a left ovarian cyst whichwas removed laproscopically. .The specimen was received as two smallfragments of a cyst wall of 3mm thickness. The microscopic appearance wasthat of a cyst wall lined by flattened, or cuboid columnar ciliated epitheliumwhich form simple papillary structures in areas. The underlying stroma waspoorly cellular , delicate and fibrillary in nature. The lining epithelium ,andstroma were strongly GFAP positive and WT1 negative. No atypical orimmature neural elements were present and there was no evidence ofmalignancy. The features are consistent with a benign cystic monodermalteratoma of neurogenic type and this is a rare case with only two reports in theliterature in the last 20 years. The appearances of ciliated epithelial lining andthe papillary choroid structures can easily be misdiagnosed as a benign orborderline serous cyst if the underlying fibrillary stroma is not appreciated.Reference:International Journal of Gyne.Pathology 1990,9:283-290Histopathology Journal 1994, 24 : 477-480P101Regulation of MUC16 in Ovarian Cancer by Micro RNAPBurns 1 , R Mezher 11 Leeds Institute of Molecular MedicineCA125 is a tumour antigen used to monitor progression and response to therapyin epithelial ovarian cancer, and is encoded by the MUC16 gene. Micro RNA’s(miRNA) are short non-coding RNA strands that can regulate gene expressionthrough regulation of the stability or translation of mRNA. We investigated thepossible regulation of MUC16 in ovarian cancer cell lines by four miRNAspredicted to target this gene.Relative quantification real time PCR was performed on mRNA extracted from22 primary ovarian tumour cell lines grown from ascitic fluid samples takenfrom 17 ovarian cancer patients. The established ovarian tumour cell lineOVCA433 was used as a calibrator. The levels of miR-92, miR-193a, miR-452and miR-651 miRNAs and MUC16 mRNA were measured relative to astandard internal control, ß-actin. MUC16 mRNA levels were found to vary bythree orders of magnitude between the 22 cell lines, compared to a variation ofonly 30-fold in the levels of CA125 found in the serum of the 17 patients at thetime of ascitic fluid removal. We found very high (> 0.8) or high (> 0.6)positive correlations of 0.869, 0.773, 0.713 and 0.690 when MUC16 mRNAlevels were compared to miR-92, miR-453, miR-193a and miR-651 levelsrespectively. These observations are consistent with the negative regulation ofMUC16 mRNA translation, rather than stability, by each of four miRNAspredicted to target this gene transcript.P100A Case of Cervical Squamous Cell Carcinoma andSchistosomiasisR Brannan 1 , N Wilkinson 11 Department of Histopathology, St James' University Hospital, LeedsWe report the case of a 26 year old female who presented with mild dyskaryosison a cervical smear. At colposcopy examination, she was thought to have highgrade cervical intraepithelial neoplasia (CIN) and a cervical biopsy wasperformed. The biopsy showed CIN II, CIN III and spherical bodies withterminal spines consistent with Schistosoma haematobium eggs. A LLETZ wasperformed and this showed high grade CIN, well-differentiated invasivesquamous cell carcinoma and further schistosome eggs.When schistosomiasis affects the female genital tract, it is most commonlyseen in the cervix and is usually Schistosoma haematobium. The presence ofSchistosoma haematobium in the bladder is known to be associated with thedevelopment of squamous cell carcinoma due to long-standing chronicinflammation and it is feasible that it plays a similar role in the cervix. Wereview the published literature looking at the role schistosomiasis of the cervixmight play in the development of cervical squamous cell carcinoma.P102The Diagnostic Utility of PCR in a SpecialistHaematopathology UnitA Silvanto 1 , ADRamsay 11 University College London HospitalMolecular analysis of tissue using the polymerase chain reaction (PCR) is animportant adjunct to diagnosis in haematopathology. PCR is used for thedemonstrating T or B cell clonality and for detecting specific chromosomaltranslocations. A PCR technique is also available for the detection oftuberculosis (TB). The diagnostic contribution of PCR in our department wasassessed by a twelve-month retrospective analysis of its use.Our haematopathology unit receives around 2300 biopsies annually. In2006 PCR was used on 118 biopsies (5%) and on 15 cytology specimens. In 19cases (14%) PCR could not be assessed due to insufficient or poor qualityDNA; this was seen most frequently in skin biopsies suspicious for mycosisfungoides (7 cases). Where DNA was successfully extracted, PCR wasperformed for suspected lymphoma in 92 cases (81%) and for TB in 22 cases(19%).In no case did TB PCR yield a positive result. In suspected lymphomaPCR made an important contribution to the final diagnosis in 84 cases (91%),and amended an initial diagnosis in 7 cases (8%). In 13 cases (14%) PCR didnot corroborate the provisional diagnosis, and was deemed non-contributory.Our results show that PCR is a valuable tool in lymphoma diagnosis, butis less helpful in identifying TB or confirming the diagnosis of mycosisfungoides in skin biopsies, where the DNA extraction can be problematic.These findings may be useful in planning the appropriate use of PCR inhaematopathology units.Summer Meeting (194 th ) 1–4 July 2008 Scientific Programme57