2008 Summer Meeting - Leeds - The Pathological Society of Great ...

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O17Regulation of the Adenomatous Polyposis Coli gene by themiR-135 family in colorectal cancerB Diosdado 1 , R Nagel 2 , C Le Sage 2 , M Van Der Waal 2 ,JAF Oude Vrielink 2 , A Bolijn 1 , GA Meijer 1 ,RAgami 21 Department of Pathology, VUmc University Medical Centre, Amsterdam,the Netherlands, 2 Division of Tumour Biology, The Netherlands CancerInstitute, Amsterdam, the NetherlandsBackground: The canonical Wnt pathway maintains the proliferativecompartment at the crypts of the intestine and cell fate and position of theepithelial cells. Inactivation of the tumour suppressor gene AdenomatousPolyposis Coli (APC), a key component of the Wnt pathway, causes formationof adenomas. MicroRNAs (miRNAs) are non-coding RNAs which silence theexpression of their target genes. MiRNAs act as oncogenes and tumoursuppressors by regulating the expression of genes involved in centralmechanisms of tumorigenesis.Aim: To determine miRNAs involved in CRC initiation by elucidatingmiRNAs regulating the Wnt pathway.Methods: A miRNA expression library was used to identify miRNAs regulatingthe APC gene. Functional relevance of the APC-miRNA interactions wasassessed using TOPFLASH reporters and transcriptional induction of c-myc.Expression of the APC gene and the miRNAs was measured by qRT-PCR in 20adenocarcinomas, 23 adenomas and 18 controls.Results: We showed that miR-135a&b target the 3’UTR of APC, suppress itsexpression and induce Wnt pathway activity. We found significant upregulationof miR-135a&b from controls, through adenomas toadenocarcinomas, which is correlated with decreasing expression of APC.Conclusion: Our results uncovered a miRNA-mediated mechanism for thecontrol of the Wnt pathway activity and suggest its contribution to CRCpathogenesis.O19Changes in Autopsy Practice Following Cardiac Surgery Over17 YearsMF Alikhan 1 , PJ Gallagher 1 , DE Pontefract 11 Department of Pathology, Southampton University Hospitals,Southampton UKBACKGROUND: There has been a decline in general autopsy frequency andthe use of histology in recent years. Previous studies at our centre havedemonstrated a constant autopsy rate (86-88%) but declining use of histologyafter cardiac surgery.AIMS: To assess changes in the autopsy rate, use of histology and cause ofdeath following adult cardiac surgery in a UK teaching hospital.METHODS/RESULTS: A retrospective analysis of the local cardiac surgerydatabase identified 5089 patients over the age of 16 who underwent a total of5885 procedures between April 2002 and October 2007. Deaths in this groupwere identified by automated database integration with Office of NationalStatistics data. Autopsy reports from this institution and surrounding hospitalswere sought. 226/5089 (4.4%) patients died prior to post-operative dischargewith 141(62%) undergoing autopsy. 307/4863 (6.3%) patients died after postoperativedischarge. In contrast to those patients who died in hospital only 21(7%) had an autopsy (p
O21Undergraduate exposure to clinical pathology and autopsies:potential effects on junior doctor recruitment intohistopathologyLBrowning 1 ,JLortan 1 , E Soilleux 11 Nuffield Department of Clinical Laboratory Sciences, Oxford UniversityThere has been a gradual erosion of clinical pathology teaching fromundergraduate medical curricula. Our medical school is the only UK medicalschool that has retained a block laboratory medicine course, which takes placeclose to the beginning of the first year of clinical training. At this medicalschool, which retains a traditional 3-year preclinical and 3-year clinicalstructure, we accept students from 3 different preclinical courses, as well asfrom our own one-year graduate entry preclinical course. These students have,therefore, had varying previous exposure to pathology.Methods: Our 161 first year clinical students were asked to complete ananonymous questionnaire at the beginning and end of the 9-week block coursedetailing their perception of the role of the histopathologist and their attitudestowards a future career in histopathology.Results: Prior to the block laboratory medicine course 142/161 (88.2%)students responded, while 124/161 (77.0%) students responded at the end of thecourse. Prior to the course, 23/142 (16.1%) students had witnessed an autopsy,119/142 (83.8%) students believed pathologists were medically qualified,91/142 (64.1%) students were sure that being a pathologist did not entailpredominantly forensic work, 88/142 (62.0%) students believed thatpathologists had an input into patient management and 87/142 (61.3%) studentsbelieved a pathologist might see live patients (e.g., in a fine needle aspirateclinic). Following the block course, 104/124 (83.9%) students had witnessed anautopsy, 117/124 (94.4%) students believed pathologists were medicallyqualified, 115/124 (92.7%) students were sure that being a pathologist did notentail predominantly forensic work, 112/124 (90.3%) students believed thatpathologists had an input into patient management and 113/124 (91.1%)students believed a pathologist might see live patients. Crucially, prior to thecourse only 6/142 (4.2%) students said they would consider a career inhistopathology, while, following the course, this figure had risen to 29/124(23.4%), p
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PROGRAMME ACKNOWLEDGEMENTSPublished
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PROGRAMME QUICK REFERENCE TABLES -
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SCIENTIFIC SESSIONS INFORMATIONSLID
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GENERAL ARRANGEMENTSSOCIAL ACTIVITI
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Detailed Programme - Tuesday 1 July
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Detailed Programme - Tuesday 1 July
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Detailed Programme - Wednesday 2 Ju
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Detailed Programme - Thursday 3 Jul
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Detailed Programme - Friday 4 July
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ACKNOWLEDGMENTSas at the time of go
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AbstractsPlenaryNote: Presenter’s
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PL5Identification of Key Breast Can
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Page 40 and 41: P33What is the Real Incidence of HE
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Page 58 and 59: P103Biopsy Pathology in HIV in the
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Page 64 and 65: P127The effect of inhaled carbon di
Page 66 and 67: P135Clinical significance of miR-21
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Page 72 and 73: P159Immunohistochemistry Biomarker
Page 74 and 75: P167Presence of stem cell traits in
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Page 95 and 96: 95Summer Meeting (194 th ) 1-4 July
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2008 Summer Meeting - Leeds - The Pathological Society of Great ...

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