2008 Summer Meeting - Leeds - The Pathological Society of Great ...

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P167Presence of stem cell traits in human pancreatic cancer celllinesNJ Guppy 1 , WR Otto 2 , MR Alison 11 Institute for Cell and Molecular Science, Barts and The London School ofMedicine and Dentistry, 2 Histopathology Unit, London Research Institute,Cancer Research UKCancer stem cells (CSCs) have been identified in leukaemia and some solidtumours and retain properties and markers of normal adult stem cells. However,a definitive stem cell remains unidentified within normal or malignant pancreas.The presence of four reported features of adult stem cells was evaluated in 4human PDAC cell lines (Panc-1, Psn1, COLO 357 and Capan-2): aldehydedehydrogenase (ALDH) activity was evaluated by flow cytometric (FACS)analysis using Aldefluor, ABC-transporter activity (Side Population analysis)was investigated by Hoechst 33342 efflux assay, and the presence of CD133and CD44 evaluated by immunocytochemistry (IHC) and FACS. The colonyformingpotentials of subpopulations thus isolated were assessed in vitro.An ALDHbright subpopulation and CD44+ cells were present in all lines. Aside population (SP) was present in Panc-1 and Capan-2 lines. CD133+ cellswere present in Panc-1, Psn-1 and COLO 357. Colony forming ability (CFA)was significantly higher for ALDHbright and SP cells versus controls in Panc-1and Capan-2 respectively. However, increased SP CFA may be artefactual, asHoechst exposure significantly affects proliferation and CFA in the non-SPfraction of Capan-2. CD133+ Psn-1 cells showed increased CFA versusunsorted controls, whilst this trend was reversed for Panc-1. CD133+ Colo357cells did not show elevated CFA but formed holoclone-like colonies, andprogeny of these CD133+ cells were heterogeneous for CD133 expression,suggestive of asymmetric division. Membranous CD44 expression did notcorrelate with enhanced CFA in any line.P169Metaplastic Ossification in Soft-tissue Angiomatosis: A CaseReport with Review of LiteratureSSen 1 , J Patel 1 ,NArsenovic 11 Lincoln County HospitalAngiomatosis is defined as a haemangioma that affects a large segment of thebody in a contiguous fashion, either by vertical extension, to involve multipletissue planes ( eg. Skin, subcutis, muscle, bone) or by crossing musclecompartments to involve similar tissue types (eg. Multiple muscles). Suchlesions usually present in the first two decades of life and have a highlycharacteristic but not totally specific histologic pattern. Histology usually showsa haphazard mixture of small and medium sized vessels, fat, connective tissueand lymphatics. Large amounts of mature fat frequently accompany theselesions; suggesting that these lesions may be more generalised mesenchymalproliferation rather than exclusive vascular lesions. But metaplastic ossificationhas not been described in these lesions. Here we present a rare example ofangiomatosis showing metaplastic bone formation.A 41-year old lady presented with swelling of the right thigh. Specimenwas a lump of fatty tissue with ellipse of skin, containing nodular grey areaswith foci of bleeding and calcified areas. Histology revealed a remarkeblevascular proliferation showing vertical extension involving skin andsubcutaneous tissue. The proliferation was a mixture of venous, cavernous andcapillary sized vessels scattered haphazardly throughout soft/ fatty tissue. Anunusual feature was mature bone formation close by the vascular proliferation.This case not only demonstrates a rare example of osseous metaplasia inangiomatosis, but also supports the contention that angiomatosis may be a moregeneralised mesenchymal proliferation.P168Helicobacter pylori infection in patients with chronicurticaria, correlation with the pathological findings in theirgastric biopsiesAAbdou 1 , E Elshayeb 2 ,AFarag 3 , N Elnaidany 41 Pathology Department, Faculty of Medicine, Menofiya University,2 Medicine Department, Faulty of Medicine, Menofiya University,3 Department of Dermatology and Andrology, Faculty of Medicine,Menofiya University, 4 Clinical Pharmacy Department, Faculty ofPharmacy, MSA UniversityBackground: Chronic urticaria is a persistent urticaria for longer than six weeksaffecting 20 % of general populations. Various infectious agents have beenreported as causes for urticaria including helicobacter pylori, which isconsidered as a common worldwide bacterial infection. Its role in inducingallergic conditions such as chronic urticaria has been suggested in some reportsand refused in others. This study aimed to assess the prevalence of HP infectionin patients with chronic urticaria and explore the possible etiopathogenetic linkbetween them.Methods: Thirty five patients suffering from chronic urticaria and ten normalcontrol individuals were subjected to upper endoscopic gastric biopsies toassess and semi-quantify HP infection and address other pathologicalabnormalities using routine Hematoxylin & Eosin staining and Giemsa staining.Results: Forty percent of control subjects and 57% of patients revealedpositivity for HP infection but the difference did not reach a significant level(P=0.47). The severity of urticarial symptoms was higher in HP positive groupthan negative one (P=0.019). Heavy bacterial colonization (p=0.008) andintense gastric inflammation (p
P171Audit of GP Histopathology Requests for Skin BiopsiesSAkram 1 , L Dunk 1 , S Milkins 21 University Hospitals of Leicester, 2 Kettering General HospitalMost histopathology departments receive numerous primary care skin biopsies.In many cases the lesions biopsied are non-serious in nature. However, some ofthe specimens will contain important/serious diagnoses. Clinical information isimportant for many histopathological diagnoses. The aim of this audit was toassess the information provided by GPs on skin biopsy request forms, todetermine what kinds of lesions GPs are removing and to compare any clinicaldiagnosis provided with the histological diagnosis.We examined 100 primary care skin biopsy request forms received inJanuary 2006 at a district general hospital. GPs were excellent at reporting thesite of the lesion but only 54% gave a description of the lesion. They were verypoor at providing any relevant clinical history and poor at reporting the type ofbiopsy performed. 41% offered a clinical diagnosis, and of these 76% werefound to have given the correct diagnosis. 18 different histological diagnoseswere made, with most being non-serious lesions. Seborrhoeic keratosis was themost commonly diagnosed lesion (29 cases), with intradermal naevus the nextmost frequent diagnosis (17 cases). All other diagnoses were made on 8 or lessoccasions. 5 basal cell carcinomas, 2 squamous cell carcinomas, and 2malignant melanomas were removed by the GPs. The malignant melanomas,squamous cell carcinoms and possibly the basal cell carcinomas should havebeen referred to a dermatologist for removal. We have made a number ofrecommendations that we hope would encourage GPs to provide all therequired clinical information.P173Comparison of Different Waxes for the Construction of TissueMicroarraysCEL Orange, KA Oien1 Division of Cancer Sciences and Molecular Pathology, Faculty ofMedicine, University of Glasgow, UKTissue microarrays (TMAs) are important in pathology research. Theirconstruction and the small size and large number of tissue cores used rendersthem more fragile than whole blocks. The type and quality of wax used to makeTMAs may reduce this fragility. Our aim was to assess different waxes tocompare their performance in TMA production.Ten waxes were tested: Histowax (Cellpath), Blue Ribbon, Histowax(Surgipath), Tissue Tek III, Lambwax, Purewax, VA5, Paraplast, Paraplast X-tra and Precision Cut. Tissue was embedded in each wax to create donor blocksand sectioning performed. Separate recipient blocks were made. TMAs wereconstructed and sections cut and stained. At each step the waxes were assessed,compared and ranked.In the generation, sectioning and staining of whole blocks, most waxesperformed well with only slight cracking. Three waxes showed some tissueseparation when sectioning. When making recipient blocks, waxes ranged infirmness, with some rather brittle or soft. In TMA construction, the most brittlewaxes impaired tissue coring. For TMA sectioning and staining, most waxesperformed well but two were difficult to section.Overall, three waxes performed consistently well, ranking in the top halffor each step assessed, particularly for the TMA-related criteria: Paraplast,Tissue Tek and Lambwax. All three contain polymers, which provide elasticityand are postulated to minimise wax cracking and separation, and are of similarcost. Although the remaining seven waxes were of good quality, we consideredthat Paraplast, Tissue Tek and Lambwax would be particularly suitable forTMA production.P172Visceral Leishmaniasis Presenting in Kaposi's Sarcoma of theSkinDKermani 1 , B Kaur 1 ,FDeroide 1 ,VSwale 1 ,SBhagani 11 Royal Free HospitalA 40 year old man - HIV positive - presented with an 11 month history ofmultiple asymptomatic purple papules, weight loss and night sweats. Histologyof the skin showed features of coexistent Leishmaniasis and Kaposi’s sarcoma.Bone marrow aspirate confirmed visceral Leishmaniasis.There is a high prevalence of visceral leishmaniasis among HIV positivepatients. In parallel with this are reports of the co-existence of leishmaniaparasites in a variety of skin eruptions including Kaposi’s sarcoma,dermatofibroma, psoriasiform dermatitis, tattoo infiltration, dermatomyositisand herpes zoster. In our patient the finding of Leishmania parasites within aKaposi sarcoma enabled a diagnosis of unsuspected visceral Leishmaniasis. Thefact that vascular lesions regressed completely after anti-protozoal therapycould support the idea Kaposi sarcoma might be a reactive condition rather thana true neoplasm.REFERENCESBosch RJ, Rodrigo AB, Sanchez P et al. Presence of Leishmania organisms inspecific and non-specific skin lesions in HIV-infected individuals with visceralleishmaniasis. Int J Dermatol. 2002 Oct;41(10):670-5.1Perrin C, Del Giudice P, Taillan B. Leishmaniasis and Kaposi's sarcoma in anHIV-infected patient.Am J Dermatopathol. 1997 Feb;19(1):101-P174A Virtual Reality Powerwall Compared to the ConventionalLight Microscope: Results of a Pilot StudyDTreanor 1 , N Jordan-Owers 1 ,JHodrien 2 , J Wood 2 , R Ruddle 2 ,PQuirke 21 Pathology and Tumour Biology, Leeds Institute of Molecular Medicine,University of Leeds, 2 School of Computing, University of LeedsBACKGROUND: Previous work presented to the Pathological society showedthat virtual slides are up to 60% less efficient than the conventional lightmicroscope. We sought to develop and test a system to improve the efficiencyof virtual slides.METHODS: A Powerwall is a novel user interface device which allows displayof large high resolution images. It comprises an array of 28 LCD screenscontrolled by an 8 node computer cluster. The resulting image is over 5 by 3metres in size, but has the same resolution as a conventional computer screen.This allows, for example, a whole gastrointestinal biopsy to be seen at once at aresolution which shows every individual cell in detail. Custom-made softwarewas written to render virtual slides on a 50 megapixel virtual reality powerwall.A controlled experiment was performed with 8 pathologist subjects and 4standard tasks to compare the efficiency and acceptability of the two modalities.RESULTS: Users had no previous experience of using a powerwall. Efficiencyand diagnostic confidence were equivalent - mean time to complete diagnostictasks was 86s for the conventional microscope and 88s for the powerwall; selfreporteddiagnostic confidence was over 90% for both modalities.CONCLUSIONS: This pilot study suggests that a powerwall has the potentialto be as efficient as the conventional microscope, and despite the lack offamiliarity of subjects with the system their diagnostic confidence wasmaintained using this new interface.Summer Meeting (194 th ) 1–4 July 2008 Scientific Programme75
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PROGRAMME ACKNOWLEDGEMENTSPublished
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PROGRAMME QUICK REFERENCE TABLES -
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SCIENTIFIC SESSIONS INFORMATIONSLID
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GENERAL ARRANGEMENTSSOCIAL ACTIVITI
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Detailed Programme - Tuesday 1 July
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Detailed Programme - Tuesday 1 July
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Detailed Programme - Wednesday 2 Ju
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Page 32 and 33: P1Distinction of Fibroadenoma & Phy
Page 34 and 35: P9Mistletoe should be reserved for
Page 36 and 37: P17Specimen Handling of Screen-Dete
Page 38 and 39: P25Mib1 Tumour Growth Fraction Asse
Page 40 and 41: P33What is the Real Incidence of HE
Page 42 and 43: P41MCPH1, a potential predictor for
Page 44 and 45: P49CTEN positively regulates cell m
Page 46 and 47: P57Meta-clustering of Small Intesti
Page 48 and 49: P65Colovesical Fistula - Complicate
Page 50 and 51: P71Ciliated foregut cyst of gallbla
Page 52 and 53: P79Evaluation of survivin expressio
Page 54 and 55: P87Incompletely Differentiated (Unc
Page 56 and 57: P95Ovarian Neuroblastoma Arising Wi
Page 58 and 59: P103Biopsy Pathology in HIV in the
Page 60 and 61: P111YY1 is a Prognostic Marker in F
Page 62 and 63: P119Ossifying Fibromyxoid Tumour of
Page 64 and 65: P127The effect of inhaled carbon di
Page 66 and 67: P135Clinical significance of miR-21
Page 68 and 69: P143Streamed Internet Video for Pat
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Page 72 and 73: P159Immunohistochemistry Biomarker
Page 76 and 77: P175Systematic Random Sampling with
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Page 84 and 85: O253 Dimensional Pathology with Vir
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Page 88 and 89: S1Virtual slides in research and di
Page 90 and 91: S9Incipient testicular germ cell ne
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2008 Summer Meeting - Leeds - The Pathological Society of Great ...

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