Dissolved organic matter in water of Daugava river
Dissolved organic matter in water of Daugava river
Dissolved organic matter in water of Daugava river
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Application <strong>of</strong> Fluorescent Pigments and Dyes <strong>in</strong> the Research <strong>of</strong> PlantStem Cells and Programmed Cell DeathM.Selga* 1 , T.Selga 2 , A.Ozol<strong>in</strong>a 11 Daugavpils University, Daugavpils, Latvia;2 Faculty <strong>of</strong> Biology, University <strong>of</strong> Latvia, Riga, LatviaE-mail: turs.selga@lu.lvDifferent types <strong>of</strong> stem cells have been stated both <strong>in</strong> plants and animals [1]. The research<strong>of</strong> stem cells particular abilities, regulation and fate becomes <strong>in</strong>creas<strong>in</strong>gly important dueto the fast development <strong>of</strong> regenerative medic<strong>in</strong>e and new methods <strong>of</strong> plant propagation.However little is known about the flower<strong>in</strong>g plant stem cell diversity, pools,developmental strategies and stemness <strong>of</strong> differentiated cells and regulation <strong>of</strong>programmed cell death [2]. Cells <strong>of</strong> tobacco (Nicotiana tabacum L.), field beans (Viciafaba L.) and other plants cultivated <strong>in</strong> optimal conditions were researched by the light andconfocal laser-scann<strong>in</strong>g microscopy. We have stated that: The asymmetric strategy <strong>of</strong> stem cell division and fate <strong>of</strong> daughter cells; Switch<strong>in</strong>g the structural mechanisms <strong>of</strong> mitosis that correlates with the reflectiveprocesses <strong>of</strong> cell differentiation and dedifferentiation; The last<strong>in</strong>g association <strong>of</strong> subord<strong>in</strong>ated unequal daughter nuclei and cells viaplasmodesmata due to arrested asymmetric mitosis; Both mitosis and asymmetric programmed cell death is double regulated – by<strong>in</strong>tr<strong>in</strong>sic and extr<strong>in</strong>sic signall<strong>in</strong>g via evident structural pathways;Plastid-nucleus complex encircles mitosis <strong>in</strong> photosynthesis<strong>in</strong>g cells and existsdur<strong>in</strong>g programmed cell death [3].Peculiarities <strong>of</strong> sta<strong>in</strong><strong>in</strong>g with fluorescent dyes <strong>in</strong> different stages <strong>of</strong> cell development willbe discussed.1. L<strong>in</strong>, H. Cell Biology <strong>of</strong> Stem Cells: an Enigma <strong>of</strong> Asymmetry and Self-renewal. J Cell Biol, 2008,vol.180, p 257-260.2. Krishnamurthy K.V., Krishnaraj R., Chozhavendan R. et al The programme <strong>of</strong> cell death <strong>in</strong> plantsand animals – A comparison. Current Sci. 2000. vol. 79, p. 1169-1181.3. Selga T., Selga M., Gob<strong>in</strong>s V., et al. Plastid-nuclear complexes: permanent structures <strong>in</strong>photosynthesiz<strong>in</strong>g tissues <strong>of</strong> vascular plants. Env and Exp Biol, 2010, vol. 8, p. 85–92.- 19 -
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