Dissolved organic matter in water of Daugava river

Synthesis and Spectroscopic Study of Benzanthrone 3-N-Derivatives asNew Highly Fluorescent DyesE.M.Kirilova 1 , A.I.Puchkin 1 , I.D.Ivanova 2 , A.Ruža 1 , G.K.Kirilov, N.Orlova 21 Chemistry Department, Daugavpils University, 13 Vienibas str., LV-5401, Daugavpils(Latvia);2 Faculty of Chemistry, University of Latvia, 48 Valdemara str., LV-1013, Riga (Latvia).e-mail: elena.kirilova@inbox.lvToday many techniques use fluorescent dyes for the labelling of biological objects. Newpractical uses call for the synthesis of new fluorescent probes with improved properties. Inpresent work, we have synthesized a number of 3-aminobenzanthrone N-derivatives –substituted amines, imines and amidines, obtaining highly fluorescent compounds [1].Synthesized dyes are the analogues of cell membrane hydrophobic probe – 3-methoxybenzanthrone,but new derivatives are long-wavelength light-emitting fluorescent dyes.YNR 1R 2YXOIIYNRNR 1R 2OIOIIIYNROIVThe spectral behaviour of the obtained dyes was investigated. We have studied thespectral properties of prepared derivatives – absorption and fluorescence spectra invarious solvents. For obtained dyes large Stokes shift values (about 100 nm) are observed,excitation maxima of the dyes are located near 500 nm and emission maxima near 650nm. In addition these compounds are showed strong fluorescent solvatochromism.It was found that many of synthesized compounds are quite sensitive to the surroundingenvironments and are potential fluorescent probes for screening structural and functionalalterations of cell membranes.- 8 -