Dissolved organic matter in water of Daugava river

Synthesis and Crystal Structure of Novel Amidino Derivatives ofBenzanthroneE.M.Kirilova 1 , I.D.Ivanova 2 , A.I.Puchkin 1 , S.V.Belyakov 31 Chemistry Department, Daugavpils University, 13 Vienibas str., LV-5401, Daugavpils(Latvia);2 Faculty of Chemistry, University of Latvia, 48 Valdemara str., LV-1013, Riga (Latvia);3 Latvian Institute of Organic Synthesis, Riga, Latvia.e-mail: elena.kirilova@inbox.lvToday many techniques use fluorescent dyes for the labeling of biological objects [1].Practical applications uses call for the synthesis of new fluorescent compounds withimproved properties for a specific requirement. In this connection the intensiveinvestigations for preparation of new fluorescent probes now have developed.In our previous investigations were synthesized a number of benzanthrone N-containingderivatives, obtaining highly fluorescent compounds [2, 3]. Synthesized dyes are sensitivelong-wavelength light-emitting fluorescent dyes, which have high photostability and lowcytotoxicity. It has been shown that some of obtained compounds are potential fluorescentprobes for screening structural and functional alterations of cell membranes and forestimation of the immune state [2].The aim of this work was the modification of some of benzanthrone derivatives (I, III) byincluding new substituents in chromophoric system in order to find potential fluorescentprobes with large luminescence intensity and high stability. Here we present the synthesisof several new benzanthrone derivatives (II, IV) with amidine group:NH 2NRNR 1R 2OIOIIBrBrN H 2OIIIRR 2R 1NNOIVSynthesized derivatives have bright from yellow to red fluorescence in organic solventsand solid state. The structure of obtained compounds was confirmed by NMR and IRspectroscopy. In addition thermal analysis and crystal structures of studied compoundshave been investigated.1. Kirilova, E. M; Kalnina, I; Kirilov, G. K; Meirovics, I. J. Fluoresc 2008, 18 (3-4), 645-648.2. Kirilova, E. M; Belyakov, S. V; Kalnina, I. Topics in Chemistry & Materials Science, Sofia:Heron Press (ed: G. Vayssilov, R. Nikolova) 2009, 3, 19-28.3. Gorbenko, G.; Trusova, V.; Kirilova, E.; Kirilov, G.; Kalnina, I.; Vasilev, A.; Kaloyanova, S.;Deligeorgiev, T. Chem. Phys. Lett, 2010, 495, 275–279.- 9 -