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CNS STANDARD OPERATING PROCEDURE Two Photon ...

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<strong>CNS</strong> <strong>STANDARD</strong> <strong>OPERATING</strong> <strong>PROCEDURE</strong>LZR001, <strong>Two</strong> <strong>Photon</strong> Fluorescence Imaging SystemFigure 10 Inside the scan head box. The dichroic mirror that separates the excitation beam from the signal beam ison the right. .................................................................................................................................................................. 14Figure 11. Gently insert Allen wrench into hole on right side of scan head to loosen the screw that holds the dichroicmirror in place. ............................................................................................................................................................ 14Figure 12 Dichroic mirror for the two photon fluorescence which is kept on the shelf in the rack of optics. Thevisible dichroic filter should be stored in a protective plastic box on this shelf when it is not in use. ........................ 15Figure 14 Changing PMT connector connections ....................................................................................................... 16SOP107Revision: 0.4Page 2 of 18 Effective Date: 04-13-2011

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