CNS STANDARD OPERATING PROCEDURE Two Photon ...
CNS STANDARD OPERATING PROCEDURE Two Photon ...
CNS STANDARD OPERATING PROCEDURE Two Photon ...
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<strong>CNS</strong> <strong>STANDARD</strong> <strong>OPERATING</strong> <strong>PROCEDURE</strong>LZR001, <strong>Two</strong> <strong>Photon</strong> Fluorescence Imaging SystemSingle <strong>Photon</strong>Fluorescence<strong>Two</strong> <strong>Photon</strong>FluorescenceFigure 2 Demonstration of the localization of the two photon fluorescence signal to the focal spot of the nearinfrared laser beam. Image borrowed fromhttp://belfield.cos.ucf.edu/one%20vs%20two-photon%20excitation.html5. Coherent Mira 900D Femtosecond/Picosecond Ti:Sa laser5.1. System overview5.1.1. Identify the parts of the laser system with the help of Figure 3.5.1.2. Contact <strong>CNS</strong> personnel immediately if there are parts missing or if the laser systemseems to be running (and it should not) or if you read any error messages on the screens.SOP107Revision: 0.4Page 7 of 18 Effective Date: 04-13-2011