<strong>HIR</strong>-MOHE EQUIPMENT <strong>2012</strong> - <strong>2013</strong> (CHANCELLORY)No PI's Name Title <strong>Equipment</strong>Estimated budgetYear 2 & 3(<strong>2012</strong> & <strong>2013</strong> )Approved Budget (After VM Lab)<strong>2012</strong> (RM) <strong>2013</strong> (RM) Total (RM)Quantity/unitCountry <strong>of</strong>originDetailsJustification <strong>of</strong>purchaseAdditional information(space/utilities eg electric, s<strong>of</strong>tware etc)Potential users/research groupsFrequency and intensity <strong>of</strong> usage8 Large bench80,000 80,000 - 80,000 1 UK/For big scale separation and characterization <strong>of</strong>To separate different types <strong>of</strong> sample size fromLab space available in <strong>HIR</strong> Building.Potential users including geneticists, molecular Frequently used (3 days per week)centrifugeUSA/macromolecule, subcellular and cellular components in microcentrifuge tube to 250ml culture tube. None existing inbiologists and microbiologists involved inGermanybiochemistry, cellular and molecular biology andthe lab. Big scale separation and characterization <strong>of</strong>animal, plant and bacteria work.medicine.macromolecule should be done immediately9 Gel documentation60,000 60,000 - 60,000 1 USA For the imaging and documentation <strong>of</strong> nucleic acid and To analyse and study DNA fragment from agarose gelLab space available in <strong>HIR</strong> Building.Potential users including geneticists, molecular Heavily used (6 days per week)systemprotein polyacrylamide or agarose gels typically stained electrophoresis. Current unit is located far away from <strong>HIR</strong>biologists and microbiologists involved inwith ethidium bromide or other fluorophores such asBuilding.animal, plant and bacteria work. Results couldSYBR Green.be published in Tier-1 journals includingImmunity, Molecular Cell, Genes &Development,Proceedings <strong>of</strong> the National Academy <strong>of</strong>Sciences <strong>of</strong> the United States <strong>of</strong> America(PNAS), PLoS Genetics, Medical Genetics andothers.10 Refrigerated55,000 55,000 - 55,000 1 UK/USA/For the separation and characterization <strong>of</strong>To separate different types <strong>of</strong> sample size fromLab space available in <strong>HIR</strong> Building.2 <strong>HIR</strong>-funded projects: (7); Regulation <strong>of</strong>Daily used ( 7 days per week)centrifugeGermanymacromolecule, subcellular and cellular components in microcentrifuge tube to 250ml culture tube but at lowImmune Response by Epigenetics and (8)biochemistry, cellular and molecular biology andtemperature. None existing in the lab. Cells need to beGenome Organization in Diseases: frommedicine at low temperature.processed immediately.Mutations to Structural Variations andEpigenetics11 Carbon dioxideIncubator12 Class II BiologicalSafety Cabinetswith HEPA-filtered13 Proteinelectrophoresis andwestern blotting set14 Thermal cycler(384 wells)50,000 - 50,000 50,000 2 USA For routine culturing <strong>of</strong> animal, human and cancer cells. A basic and standard equipment for routine cell culturing and Cell culture room will be setup in <strong>HIR</strong> Building. 2 <strong>HIR</strong>-funded projects: (7); Regulation <strong>of</strong> Daily used ( 7 days per week)conducting cell-based experiments. Current CO2 incubator inImmune Response by Epigenetics and (8)the lab is fully utilized and not sufficient to meet requirementGenome Organization in Diseases: from<strong>of</strong> project. To minimize contamination, we need new units inMutations to Structural Variations andthe <strong>HIR</strong> building.Epigenetics60,000 60,000 - 60,000 2 USA An enclosed, ventilated laboratory workspace for safely As tissue culture work is prone to contamination, it is Cell culture room will be setup in <strong>HIR</strong> Building. 2 <strong>HIR</strong>-funded projects: (7); Regulation <strong>of</strong> Heavily used (6 days per week)handling <strong>of</strong> cells and conducting cell-basedessential to have a dedicated facilities.This system isImmune Response by Epigenetics and (8)experiments.important for collaborative work with Caltech. None in theGenome Organization in Diseases: from<strong>HIR</strong> Building.Mutations to Structural Variations andEpigenetics.100,000 100,000 - 100,000 2 USA For separating proteins ranging in size from 5 to 2,000 To separate proteins according to their electrophoretic Lab space available in <strong>HIR</strong> Building.2 <strong>HIR</strong>-funded projects: (7); Regulation <strong>of</strong> Heavily used (6 days per week). Each run willkDa due to the uniform pore size provided by the mobility (function <strong>of</strong> the length <strong>of</strong> a polypeptide chain and itsImmune Response by Epigenetics and (8) take about 30 min to a few hours.polyacrylamide gel and blotting <strong>of</strong> the protein to charge) and to blot it to membrane for protein detection.Genome Organization in Diseases: frommembraneExisting facility is not enough to meet the requirement <strong>of</strong> theMutations to Structural Variations andproject.Epigenetics. Results could be published in Tier-1 journals includingImmunity,Molecular Cell, Genes &Development,Proceedings <strong>of</strong> the National Academy <strong>of</strong>Sciences <strong>of</strong> the United States <strong>of</strong> America(PNAS), PLoS Genetics, Medical Genetics andothers40,000 40,000 - 40,000 1 USA For amplifying segments <strong>of</strong> DNA via the polymerase To conduct high throughput research studies in gene Lab space available in <strong>HIR</strong> Building. Can be placed 2 <strong>HIR</strong>-funded projects: (7); Regulation <strong>of</strong> Heavily used ( at least 3 days per week). Eachchain reaction (PCR) process.expression, mutation, and sequencing. None existing. in Institute <strong>of</strong> Biological Sciences labImmune Response by Epigenetics and (8) run will take about 30 min to a few hours.Genome Organization in Diseases: fromMutations to Structural Variations andEpigenetics. Results could be published in Tier-1 journals includingImmunity, Molecular Cell, PLoS Genetics,Medical Genetics. Other potential usersincluding UM Scientists working in drugdiscovery, genetics, pharmacogenomics,bacterial, molecular biology and cancer work.15 Centrifuge 30,000 30,000 - 30,000 2 USA For the general separation and characterization <strong>of</strong>For a quick, easy, and economical way to spin down microLab space available in <strong>HIR</strong> Building.2 <strong>HIR</strong>-funded projects: (7); Regulation <strong>of</strong>Heavily used (6 days per week).macromolecule, subcellular and cellular components in samples, perform micr<strong>of</strong>iltration work and cell separations.Immune Response by Epigenetics and (8)biochemistry, cellular and molecular biology andGenome Organization in Diseases: frommedicine.Mutations to Structural Variations andEpigenetics.16 Autoclave machine 30,000 30,000 - 30,000 1 Japan An instrument to sterilize equipment and supplies byMost <strong>of</strong> the equipment and supplies must be sterilized before Lab space available in <strong>HIR</strong> Building.2 <strong>HIR</strong>-funded projects: (7); Regulation <strong>of</strong>Heavily used (6 days per week).subjecting them to high pressure saturated steam.conducting an experiment. Besides, all biohazard wasteImmune Response by Epigenetics and (8)materials produced from the experiment must be sterilizedGenome Organization in Diseases: frombefore discarding. Current unit (1) in the lab is fully utilizedMutations to Structural Variations andand not sufficient to meet requirement <strong>of</strong> project.Epigenetics.17 Chromatography Refrigerator 25,000 25,000 - 25,000 2 USA For storage <strong>of</strong> chemicals, DNA, reagents andTo store chemical, DNA, reagents and to conduct experimentLab space available in <strong>HIR</strong> Building.2 <strong>HIR</strong>-funded projects: (7); Regulation <strong>of</strong>Daily used ( 7 days per week)conducting experiment at 4°C.that required 4°C .Immune Response by Epigenetics and (8)Genome Organization in Diseases: fromMutations to Structural Variations andEpigenetics.18 Gel dryer 10,000 10,000 - 10,000 1 USA For drying polyacrylamide gels To dry the polyacrylamide gel from SDS-PAGE. NoneLab space available in <strong>HIR</strong> Building.2 <strong>HIR</strong>-funded projects: (7); Regulation <strong>of</strong>Frequently usedexisting.Immune Response by Epigenetics and (8)Genome Organization in Diseases: fromMutations to Structural Variations andEpigenetics.19 Micro-Volume Full-SpectrumFluorospectrometer100,000 100,000 - 100,000 1 <strong>High</strong>ly accurate measurment <strong>of</strong> DNA, RNA and protein Routinely needed for the required project, and for measuring Lab space available in <strong>HIR</strong> Building.concentrations utilizing micro volume amounts. precious amounts <strong>of</strong> DNA, RNA and protein accurately. Minor renovation, furnishing & maintenance.None existing.2 <strong>HIR</strong>-funded projects: (7); Regulation <strong>of</strong> Heavily used (6 days per week). Each time willImmune Response by Epigenetics and (8) take about about 30 min to a few hours.Genome Organization in Diseases: fromMutations to Structural Variations andEpigenetics. Other potential users includinggeneticists, molecular biologists, biochemists,and microbiologists.20 Sonicator 50,000 50,000 - 50,000 1 Rapid disruption <strong>of</strong> cells for DNA, RNA and protein Routinely needed for the required project, The use <strong>of</strong> a Lab space available in <strong>HIR</strong> Building.2 <strong>HIR</strong>-funded projects: (7); Regulation <strong>of</strong> Frequently used.extraction and purification. Also for homogenization. sonicator for cell disruption minimizes contamination, andImmune Response by Epigenetics and (8)allows faster work flow. None available.Genome Organization in Diseases: fromMutations to Structural Variations andEpigenetics. Other potential users includinggeneticists, molecular biologists, biochemists,chemists and microbiologists.21 COBAS Taqman 48360,000 - 360,000 360,000 1 USA COBAS Taqman 48 Analyzer is an automated system COBAS Taqman 48 Analyzer is one <strong>of</strong> the most versatile realtimeamplification and detection system <strong>of</strong> DNA or RNA in in the <strong>HIR</strong> Building and approved by the civil, biologists and microbiologists involved in real-will take at least 3 hours.Lab space (13'x13' with air-con) has been allocated Potential users including geneticists, molecular Heavily used (6 days per week) and each runAnalyzerfor real-time amplification and detection <strong>of</strong> DNA or RNA.Up to 2 medium-throughput assays can be performed in the market. This equipment is not available in UM despite mechanical & electrical departments. AMPLILINK time amplification, detection and quantitation <strong>of</strong>the same footprint, allowing various protocols to be the high demand for such real-time amplification facility. For operational s<strong>of</strong>tware is included for total automated DNA or RNA. At least 10 PhD and MScused at the same time. The COBAS system is also instance, the Molecular Genetics team is embarking on a solution. Without this instrument, genetic projects students will be trained using the instrument,highly compatible with various FDA-approvedlarge-scale sequencing study on respiratory, gastrointestinal, requiring an automated system for real-time producing results that could be published inRNA/DNA kits, for example, the HIV, HCV and the HBV central nervous system, and blood-borne pathogens. amplification and detection <strong>of</strong> DNA or RNA will be Tier-1 journals including Nature, Science,qualitative/quantitative assays, <strong>of</strong>fering the optimal Availability <strong>of</strong> COBAS Taqman 48 Analyzer is essential and negetively affected.Lancet, PLoS, PNAS, Journal <strong>of</strong> Virology,solutions for clinical diagnostics and research. timely to produce tier-1 publications (J Clin Microbiol. 2011Emerging Infectious Diseases and others.Jan;49(1):377-9, J Clin Microbiol. 2005 Jul;43(7):3504-7).5,500,000.00 5,090,000.00 410,000.00 5,500,000.00- updated on 2nd March <strong>2012</strong> -
<strong>HIR</strong>-MOHE EQUIPMENT <strong>2012</strong> - <strong>2013</strong> (CHANCELLORY)No PI's Name Title <strong>Equipment</strong>3 Assoc. Pr<strong>of</strong>. Dr. TunkuAtomic forceStem Cell 1Kamarul ZamanmicroscopyEstimated budgetApproved Budget (After VM Lab)Quantity Country <strong>of</strong>Justification <strong>of</strong>Additional informationYear 2 & 3Details/unitoriginpurchase(space/utilities eg electric, s<strong>of</strong>tware etc)(<strong>2012</strong> & <strong>2013</strong> )<strong>2012</strong> (RM) <strong>2013</strong> (RM) Total (RM)3,500,000 3,500,000 - 3,500,000 1 Korea AFM is used to measure the surface topography and to AFM finds various application from tissue to scaffold engineeering. Space is available at Molecular Biotechnology lab. Theinvestigate the surface forces on the nanoscale level (from The resolution <strong>of</strong> image generated is <strong>of</strong> very good quality for high s<strong>of</strong>tware for image analysis and a desktop computer isAngstroms to 100 microns) <strong>of</strong> the biological samples, impact publications. This instrument is superior over other model also included with this equipment.nan<strong>of</strong>oibres, and engineered scaffolds. Can serve as a tool to and contains both contact and non-contact imaging.study the mechanical properties <strong>of</strong> individual cells andadhesion forces between the cells, a wide variety <strong>of</strong> cellmechanism from cell development, cytoskeletal changes andapotosis can be probed and visualized. Currently AFMtechnology is rapidly adding new capabilities to detect variousphysical properties and manipulating biological entities.3,500,000.00 3,500,000.00 0.00 3,500,000.00Potential users/research groupsFrequency and intensity <strong>of</strong> usageUsers <strong>of</strong> AFM includes: biologist, pharmacologist, Used 2 days per week and each live cell imagingmolecular biologist and medical scientist performing experiment will run upto 48 hours.live cell imaging and ion conductance study.Good quality images obtained through live cellimage and surface topography can be used for Tier 1Publications.4Dr C H Raymond OoiQuantum and LaserScience1 Spectrometer anddetecter100,000 100,000 - 100,000 1 Singapore <strong>High</strong> resolution spectrometer that can resolve vibrational spectra <strong>High</strong> resolution spectrometer that can resolve vibrational spectra2 Ultrashort pulsedetection system3 Laser diode andcontroller80,000 80,000 - 80,000 1 Singapore Characterization <strong>of</strong> ultrashort laser pulse Not available. Necessary for ultrafast optical pulse20,000 20,000 - 20,000 1 Malaysia For probing and controlling sample Necessary for probing sample in multiphoton parametricexcitations4 Tunable laser sources 50,000 50,000 - 50,000 1 Singapore Tuning frequency across atomic/molecular resonances Not available. Needed for studying the effects <strong>of</strong> frequency at and<strong>of</strong>f resonance.5 Optical detector /CCD 10,000 10,000 - 10,000 1 Malaysia Camera can display high resolution images rapidly Not available. Needed for obtaining microscopic images6 Single photondetectors7 Optical amplifiers/oscillator10,000 10,000 - 10,000 1 Singapore For sensitive detection <strong>of</strong> few photons Not available, necessary for detecting studying quantum properties40,000 40,000 - 40,000 1 Singapore For amplifying laser pulse Not available. Needed for amplifying laser pulses for nonlinearoptics processes8 Optical table 100,000 100,000 - 100,000 1 Singapore For anti-vibration and stable optics Needed as a platform for optical experimentation9 Autocorrelator andcross correlator10 Acousto opticmodulator /SLM10,000 10,000 - 10,000 1 Malaysia For correlation measurement Not available. Necessary for photon correlation measurement10,000 10,000 - 10,000 1 Malaysia For creating sidebands in spectra Not available. Necessary modulating signal and making newfrequencies11 Stern Geriach magnet 5,000 5,000 - 5,000 1 Singapore For separating particles in different quantum states Not available. Needed for testing Maxwell demon concept andquantum thermodynamics12 Microwave/maserand cavity5,000 5,000 - 5,000 1 Singapore For inducing Zeeman coherence Not available. Necessary for controlling quantum coherence <strong>of</strong>statesWe are initiating quantum optics and nonlinear opticsresearch at atomic and molecular levels never beenexplored in Malaysia before. It is a major step forward,leading to futuristic optical technologies that incorporatequantum effects. The measurement equipments andoptics are different from those in photonics and opticalcommunications. This effort will produce high impact Tier1 papers and enables Malaysia to acquire the rapidlyadvancing state-<strong>of</strong>- the-art quantum science andtechnology.<strong>Research</strong>ers from quantum and ultrafast laserscience group will assemble the equipments forconducting novel research ideas. PIs andcollaborators in UM, postgraduate students, andpostdoc will be the users who will benefit from theequipments for studying quantum properties <strong>of</strong>photons and spectroscopic properties <strong>of</strong> atoms andmolecules. These equipments will enable UM todevelop the state <strong>of</strong> the art expertise in quantumand laser research, and promote interdisciplinaryresearch topics that will produce publications in Tier1 journals such as Physical Review Letters, NaturePhotonics. At least 5 PhD and 3 MSc students will betrained using these instruments.These ceqyipments are connected to construct noveloptical experimentations involving photons andatoms. They will be used regularly.13 Gas/vapor cell 5,000 5,000 - 5,000 1 Malaysia For high precision control <strong>of</strong> quantum states for newquantum optics effectsNot available. The narrow natural linewidth <strong>of</strong> gas is ideal forexploring quantum optics techniques without using solid materials14 Prisms, mirrors,gratings, beamsplitter, polarizers100,000 100,000 - 100,000 1 Malaysia For amplifying stretching and compression <strong>of</strong> ultrashort laser Not available. The components are necessary for amplifying andpulsesshaping the spectral properties <strong>of</strong> laser pulses545,000.00 545,000.00 0.00 545,000.00- updated on 2nd March <strong>2012</strong> -
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