Summary of HIR Equipment (2012-2013) - High Impact Research

HIR-MOHE EQUIPMENT 2012 - 2013 (CHANCELLORY)No PI's Name Title EquipmentEstimated budgetYear 2 & 3(2012 & 2013 )Approved Budget (After VM Lab)2012 (RM) 2013 (RM) Total (RM)Quantity/unitCountry oforiginDetailsJustification ofpurchaseAdditional information(space/utilities eg electric, software etc)Potential users/research groupsFrequency and intensity of usage6 Prof. Yip Cheng HarBreast CancerITL Lab :-1 Basic lab equipmentand freezers616,500 - - 0 1 Various sources These basic equipment for the ITL are lsited separately in Appendix Space will be in the paediatric lab in the new paediatric1 and are required to set up the labtower, UMMC. The HIR Breast project, together withUMCRI, will set up a research pathology lab, and requirethese basic equipment for the set up. The researchpathology lab can be also used by others.Ceria, Gynaeoncology.Full time, with 2 post docs2 Violet laser 110,000 110,000 - 110,000 1 USA The current FLOW can only tag 6 colours and further laser tocharacterize the cellsResearch Pathology Lab :-3 Automatic IHCStainer (VentanaDiscovery)4 Microtome (ThermoScientific)500,000 500,000 - 500,000 1 Switzerland The automated IHC system is recommended as it generates a moreconsistent and reproduce able staining patterns both within andbetween histological batches (UKNEQAS).80,000 80,000 - 80,000 1 USA The compact research microtome has extraordinary high stabilityallowing quick trim feature at 10-30 microns and a section counter.5 Cryostat120,000 120,000 - 120,000 1 USA 'The basic cryostat is for processing snap frozen research samples.(Thermoscientific)Space will be in the paediatric lab in the new paediatric6 Fumehood (ESCO) 35,000 35,000 - 35,000 1 Singaporetower, UMMC. The HIR Breast project, together with7 Paraffin embedder80,000 80,000 - 80,000 1 USA The automated paraffin embedder allows individual programing of UMCRI, will set up a research pathology lab, and require(Thermoscientific)all temperatures and automatic consideration of pre-heating times these basic equipment for the set up. The researchThe breast, colorectal, urology and gynaecologicalallowing for consistency.pathology lab can be also used by other research groups.cancer teams all have projects requiring the use of8 Shaking incubator30,000 30,000 - 30,000 1 KoreaBesides immunohistochemistry work, this is a diagnosticimmunohistochemistry. This equipment will have(Labtech)and translational facility where transfection and functional9 Light microscope with200,000 200,000 - 200,000 1 hungary The microscope with digital slide scanning capabilities (virtual slidemultiple users.assays will be carried out. Current pathology labs are useddigital slide scanningscanner) enables fully automated screening of slides resulting in for service, a dedicated lab would allow for more lab timecapabilities (Onemore accurate images. It will enhance collaborative efforts between for research.Biotech)different research unit which involves Pathology. it will also allow Minor renovation, furnishing & maintenance.digital images to be sent for pathological review without necessarilysending off slides or having a pathologist on site. The PCRthermocycler and shaking incubator are for translational workinvolving transfections and identifications of over expressedproteins.10 Tissue arrayer 80,000 80,000 - 80,000 1 USAFull time, with visiting Professor Dr Anthony Roades11 Dessicator incupboard x 2Central Medical Genetics Lab :-40,000 40,000 - 40,000 1 USA12 384 well PCR200,000 200,000 - 200,000 1 USA Thermal cycler is for amplifying segments of DNA, cDNA via These items will complement the second generation sequencermachinethe polymerase chain reaction (PCR) process as well as that will be acquired for the Central Lab Facilities in Bagunan HIR.performing Taqman array screening.13 Real-time PCR 320,000 320,000 - 320,000 1 Switzerland Real-time PCR system provides a platform for the detection To conduct high throughput research studies in gene expression, Minor renovation, furnishing & maintenance.and quantification of nucleic acid sequences. It is an ideal mutation, and quantification. The existing facility is not enough tosystem for absolute quantification and allelic discrimination meet the requirement of the project.through Taqman or Invader chemistry screening.14 Liquid handler 100,000 100,000 - 100,000 1 USA Multi-channel automated pipettes are essential when Use of automated pipettors would also ensure consistent andperforming high throughput PCR or ELISA screening. A reproducible results in volume sensitive experiments such as ELISA,calibrated automatic pipettor would eliminate inconsistencies PCR, Taqman assay screening.due to human error.15 DNA extractor 150,000 150,000 - 150,000 1 USA Current systems utilize a cartridge format or column basedtechnology to extract DNA from all sorts of samples.Preloaded buffers would ensure consistency and good qualityDNA.16 freezers -20 & -80 150,000 150,000 - 150,000 1 Japan DNA, protein and RNA samples are heat sensitive and These 3 - tems are required for storage of blood and tissuedegrade easily without proper storage. As samples are scarce samples, and the computer servers are to store the 'data,and hard to come by, the freezers would be essential in particularly large datasets generated from the next generationmaintaining sample integrity, an important preceding step sequencing technology.towards high quality and reproducible results.Tracing and locating samples can be an ardous 17 Freezerworks - for50,000 50,000 - 50,000 1 USA task in the Automated extraction of DNA would greatly speed up otherwisearchiving samplesevent of a huge amount of samples. Hence an automated laborious task of extracting DNA. A walk away set up wouldsoftware for tagging and locating samples would enable swift increase productivity and maintain consistency.retrieval of samples and systematic archiving for long termstorage.18 Computer servers 230,000.00 230,000.00 - 230,000 1 Malaysia Myriad of data generated from Next generation sequencers, Needed for analysis of very high volume of data, tracking of records,DNA array screening machines requires a powerful computing monitoring work progress and generation of research outputs forsystem to screen and analyse data. Existing do not have the publication. This will be used continuously and will form a commonnecessary computing power to meet the requirements of facility for many researchers.whole genome screening approaches. Hence a localizedserver is needed.With these equipment, we hope to set up a MedicalGenetics Lab that will cater to all the researchgroups besides the HIR Breast Genetics Programmethat require genetic testing eg the colorectal cancergroup.These equipments will be used daily and fully utilisedwhen research is in progress. Other researchers fromother groups (molecular genetics, molecular biology,biochemistry etc) will find the facility useful too fortheir research.The usage is a permanent feature.Telomerase Research :-19 FISH microscope with350,000 350,000 - 350,000 1 Japan Measurement of telomere lengths These pieces of equipment are to bring telomerase research to Will be housed in research labs in the pathologyautomated XYZ stagemore frontline level as methodologies have changed.department. Software for analysis of probe distances will(stacker)(1) The FISH scope with stacker is essential as the new method for have to be acquired and will be unique to each project.telemere length determination involves fluorescent probes andThe FISH scope and spectrophotometer can support20 Spectrophotometer 40,000 40,000 - 40,000 1 Germany Telomerase detection and quantitationmeasuring distances between signals. The human hand is not ablemany other projects that use flourescent probes andto manuveur such fine distances without an automated stage. Alsoenzymatic assays. This can encourage many f uturenumerous images have to be captured which are too laborousMaster and Doctorate studies.without automation.(2) The spectrophotometer is essential in the "TRAP" enzymaticassay for telomerase detection and quantitation.21 Ultrapure (millipore)25,000 25,000 - 25,000 1 USAFull time, with 2 scientific officers.water filtrationsystem22 Refrigerated30,000 30,000 - 30,000 1 DenmarkcentrifugeEquipment for general molecular research23 Laminar flow cabinet 30,000 30,000 - 30,000 1Will be housed in research labs in the pathologyUK General use in molecular researchWill be a shared facility.department.- existing equipment are not economical to repair.24 Thermomixer 20,000 20,000 - 20,000 1 Germany25 Thermocycler (ABS) 30,000 30,000 - 30,000 1 USA3,616,500.00 3,000,000.00 0.00 3,000,000.00- updated on 2nd March 2012 -