Summary of HIR Equipment (2012-2013) - High Impact Research

HIR-MOHE EQUIPMENT 2012 - 2013 (FACULTY OF MEDICINE)No PI's Name Project Title EquipmentsEstimated BudgetYear 2 & 3(2012-2013)2012(RM)Approved Budget(After VM Lab)2013(RM)Total(RM)QuantityCountry ofOriginDetailsJustificationAdditional information(space/utilities eg electric, software etc)Potential users/researchgroupsFrequency andintensity of usage1 Prof. Dr. AdeebaKamarulzamanMitigating the malayasian HIVepidemic through acomprehensive researchprogram(CERIA)1 E3--20c Freezer 14,000 14,000 - 14,000 1 USA -20 and -80 freezers are required for the storage of clinicalMany additional staff (RA and students) have been employed to work on the various projects Lab space available to accomodate these freezers.specimens.under the HIR grant. We need to provide these personnel with properly equipped workingenvironment which includes computing hardware for data analysis and additional labworkbenches . These equipment/items by themselves will not lead to Tier 1 publications but are2 E4--80C Freezer 71,000 71,000 - 71,000 1essential to create a condusive working environment that will encourage good research outputs.3 E16-CO2 Incubator 25,000 25,000 - 25,000 1 USA To stabilise CO2 environment which is essential for cell culture work. This equipment is essential for cell culture work which is required for a number of assays related Lab space available in ITL to accomodate the incubator.to the HIR projects. We currenly only have a single incubator which cannot accommodate theadditional projects and experiments. The lack of an additional incubator will significantly impedethe progress of our experiments and affect the ability to publish our results in a timely manner.2 Prof. Dr. SazalyA.BakarGenomics and molecularcharacterization of TropicalInfectious (TIDREC)4 E29-Luminex 450,000 450,000 - 450,000 1 USA Luminex system is a multiplexing platform capable of performing qualitative Multiple projects in CERiA need the measurement of various cytokines which is an essential Lab space available in ITL.and quantitative analysis of proteins and nucleic acids in a variety of sample parameter in all immunological studies. Without them, explanation of immunological mechanismswill merely be a speculation and will significantly impact our ability to publish our research in tiermatrices, greatly reducing input of precious sample, reagents and labor1 journals.while improving productivity.Various office equipment including 5 E31-Benches, Filing Cabinets, Sinks, etc 1,500 0 - 0 1 VARIOUSdesktop and laptop computers, Many additional staff (RA and students) have been employed to work on the various projectsprinters with scanning and fax capability). Additional lab benches and under the HIR grant. We need to provide these personnel with properly equipped workingsinksenvironment which includes computing hardware for data analysis and additional lab8,000- 8,0006 E21-Workstation HP2200 5,000 1workbenches . These equipment/items by themselves will not lead to Tier 1 publications but are7 E34-Desktop Computers 6,000 1essential to create a condusive working environment that will encourage good research outputs.8 E32-Printer/fax/Scanner 1,000 1,000 - 1,000 19 E35-laptop Computers 9,000 6,000 - 6,000 110 E36-Software 9,000 0 - 0 1 Software include SPSS (full package), GraphPad Prism, Gatelogic, These softwares are essential for data analysis and statistical analysis. Many of our research Most of the data analysis softwares are provided free only in the first yearFACSDiva, Trustport encryption and antivirus software.databases contain confidential patient information. Therefore there is a need for an additional and need to be subsequently purchased.level of security with antivirus and encryption softwares for the computers and portable storagedevices that hold these data.591,500 575,000 0 575,000450,000 450,000 - 450,000 1 E3-RTCA Realt time cellular analysis for HVPs 1 Switzerland For real-time study of cell impedance. It allows real-time monitoring of cellular processes occurring Comparable equipment is in Pharmacology but not in biocontainmentimmediately upon virus infection. This will then provide information to allow the user to make informed facility. This equipment is dedicated only for drug discovery studies and nodecisions on the downstream experiments e.g. timing of certain manipulations or treatments. provision for infectious disease work. The exiting equipment is firstgeneration model with no possibility for upgrading. Besides that, the exitingAdditionally, it provide important quantitative information on the biological status of the cells includingequipment is only for drug discovery studies. So the equipment that wecell number, viability and morphology.purchase will enable work in infectious disease for real-time examinationwhich will then provide information to allow the user to make informeddecisions on the downstream experiments e.g. timing of certainmanipulations or treatments.For real-time multianalytes screening upon virus infection belonging to Risk2 E4-Cellomic HTS for multianalyte cell content750,000 750,000 - 750,000 1 US For real-time multianalytes screening. It provides knowledge about targets and compounds of interestanalysis for HPVsin the context of the cell. The results obtained will allow the user to understand the biological processes Group 3 pathogens.. There are at least 11 researchers that could use thisoccuring, which will then add to the knowledge of targets and pathways of interest.equipment if it is equipped for infectious disease.3 E11-Liquid Nitrogen Tank 10,000 5,000 5,000 10,000 1 Malaysia For long-term storage of cells and samples1,210,000 1,205,000 5,000 1,210,0003 Prof. Dr. Hany Ariffin Cancer research in Malayasian 1 E20-AutoMACS Pro Cell Separator 250,000 230,000 - 230,000 1 Germanypopulation(UMCRI)(Miltenyi Biotec)This equipment will primarily be used for cell culturing purposes. Prior to the main step, proteins andvarious cells must first be purified and isolated for culturing purposes. This appliance enablespurification of mRNA and cDNA synthesis as well as genome related works. The cell separator alsofunctions as a stem cell purifier. as mentioned earlier, with the main use mostly associated with cellculture activities, probability of sharing this equipment is highly unlikely due to the existent risk ofcontamination by cancer cellsThe equipment is of low maintenance and requires no specific software torun on. Space is readily available currently for placement of the appliance inthe laboratory.4 Prof. Dr. Onn HajiHashimThe use of acute phasereactant proteins as cancerbiomarkers (UMPCR)2 E24-Pipettes 7,500 7,500 - 7,500 1 MalaysiaLiquid handling(Eppendorf)257,500 237,500 - 237,5001 E1-Protein Array Scanner 330,000 330,000 - 330,000 1 The scanner provides flexibility and reproducibility for a range of diverse This equipment is necessary to address the objectives of the proposed project. The new advance No issue with space and electric requirement. The image analysis software ismicroarray applications and format sizes. It is automated, high throughput, technology of protein microarray provides a wide variety of proteomics application, which includes: included with the whole system.and includes numbers of flexible features, including parallel dual-channel micro multi-analyte immunoassays, protein-antibody, antibody-protein, antibody-antigen, proteinprotein,and protein-drug microarray assays. The open system also allows acquisition of data anddetection and the ability to operate with a wide excitation range and a hugeselection of additional fluorescence emission filters. The scanner comes expression analysis from a wide range of gene array chips (whole human/mouse genome or specificwith inbuilt image analysis software, which supports its flexibility,group of genes). Analysis of multiple assays in a single run could greatly reduce the time consumption,automation and batch processing capabilities.number of sample and cost compared to conventional method. Protein Array also benefit from lessercross reactivity of antibody binding since each spot contains only single type of antibody. The systemwill be particularly useful for genomics and proteomics researchers within the Faculty of Medicine andUniversity of Malaya.5 Prof. Dr. Mohd RaisMustafaMolecular mechanisms ofdrug actions330,000 330,000 - 330,0001 E4-Real time PCR 200,000 200,000 - 200,000 1 USA Real-time PCR machine will be used to perform genes amplification which is Currently, 4 units is being purchased by FOM to cater to various research groups in variousThis equipment requires minimal maintenance and space is available at thea common method used in all molecular work for validation and real-time departments of FOM. In view of the increased in number of research groups and postgraduate students department for equipment placement.quantification of genes expression. This method is expected for any good in FOM. This number is still inadequate because one run takes 4 hours, which means in a day, at mostpublication and many researchers now have replaced the traditional semiquantitativethe equipment can do about 4 runs, hence, only 1 student from 1 research group can use 1 equipmentmethod with real-time quantitation.at a time, considering that the student will need the initial 2 runs to optimize amplificationparameters. In many experiments, usually a minimum of 5-10 genes are studied and this can alsoinvolves 100-200 patient samples such as that in pharmacogenomic studies. Hence, there is a need foran additional unit in Pharmacology department, not only to cater to the molecular work of this projectbut also to be used for other research groups in the department. Without this equipment, there will bea long queue to use the present number of equipment and this will delay the experiment and may alsojeopardize sensitive samples.200,000 200,000 - 200,000- updated on 2nd March 2012 -