Summary of HIR Equipment (2012-2013) - High Impact Research

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HIR-MOHE EQUIPMENT 2012 - 2013 (FACULTY OF MEDICINE)No PI's Name Project Title EquipmentsEstimated BudgetYear 2 & 3(2012-2013)2012(RM)Approved Budget(After VM Lab)2013(RM)Total(RM)QuantityCountry ofOriginDetailsJustificationAdditional information(space/utilities eg electric, software etc)Potential users/researchgroupsFrequency andintensity of usage1 Prof. Dr. AdeebaKamarulzamanMitigating the malayasian HIVepidemic through acomprehensive researchprogram(CERIA)1 E3--20c Freezer 14,000 14,000 - 14,000 1 USA -20 and -80 freezers are required for the storage of clinicalMany additional staff (RA and students) have been employed to work on the various projects Lab space available to accomodate these freezers.specimens.under the HIR grant. We need to provide these personnel with properly equipped workingenvironment which includes computing hardware for data analysis and additional labworkbenches . These equipment/items by themselves will not lead to Tier 1 publications but are2 E4--80C Freezer 71,000 71,000 - 71,000 1essential to create a condusive working environment that will encourage good research outputs.3 E16-CO2 Incubator 25,000 25,000 - 25,000 1 USA To stabilise CO2 environment which is essential for cell culture work. This equipment is essential for cell culture work which is required for a number of assays related Lab space available in ITL to accomodate the incubator.to the HIR projects. We currenly only have a single incubator which cannot accommodate theadditional projects and experiments. The lack of an additional incubator will significantly impedethe progress of our experiments and affect the ability to publish our results in a timely manner.2 Prof. Dr. SazalyA.BakarGenomics and molecularcharacterization of TropicalInfectious (TIDREC)4 E29-Luminex 450,000 450,000 - 450,000 1 USA Luminex system is a multiplexing platform capable of performing qualitative Multiple projects in CERiA need the measurement of various cytokines which is an essential Lab space available in ITL.and quantitative analysis of proteins and nucleic acids in a variety of sample parameter in all immunological studies. Without them, explanation of immunological mechanismswill merely be a speculation and will significantly impact our ability to publish our research in tiermatrices, greatly reducing input of precious sample, reagents and labor1 journals.while improving productivity.Various office equipment including 5 E31-Benches, Filing Cabinets, Sinks, etc 1,500 0 - 0 1 VARIOUSdesktop and laptop computers, Many additional staff (RA and students) have been employed to work on the various projectsprinters with scanning and fax capability). Additional lab benches and under the HIR grant. We need to provide these personnel with properly equipped workingsinksenvironment which includes computing hardware for data analysis and additional lab8,000- 8,0006 E21-Workstation HP2200 5,000 1workbenches . These equipment/items by themselves will not lead to Tier 1 publications but are7 E34-Desktop Computers 6,000 1essential to create a condusive working environment that will encourage good research outputs.8 E32-Printer/fax/Scanner 1,000 1,000 - 1,000 19 E35-laptop Computers 9,000 6,000 - 6,000 110 E36-Software 9,000 0 - 0 1 Software include SPSS (full package), GraphPad Prism, Gatelogic, These softwares are essential for data analysis and statistical analysis. Many of our research Most of the data analysis softwares are provided free only in the first yearFACSDiva, Trustport encryption and antivirus software.databases contain confidential patient information. Therefore there is a need for an additional and need to be subsequently purchased.level of security with antivirus and encryption softwares for the computers and portable storagedevices that hold these data.591,500 575,000 0 575,000450,000 450,000 - 450,000 1 E3-RTCA Realt time cellular analysis for HVPs 1 Switzerland For real-time study of cell impedance. It allows real-time monitoring of cellular processes occurring Comparable equipment is in Pharmacology but not in biocontainmentimmediately upon virus infection. This will then provide information to allow the user to make informed facility. This equipment is dedicated only for drug discovery studies and nodecisions on the downstream experiments e.g. timing of certain manipulations or treatments. provision for infectious disease work. The exiting equipment is firstgeneration model with no possibility for upgrading. Besides that, the exitingAdditionally, it provide important quantitative information on the biological status of the cells includingequipment is only for drug discovery studies. So the equipment that wecell number, viability and morphology.purchase will enable work in infectious disease for real-time examinationwhich will then provide information to allow the user to make informeddecisions on the downstream experiments e.g. timing of certainmanipulations or treatments.For real-time multianalytes screening upon virus infection belonging to Risk2 E4-Cellomic HTS for multianalyte cell content750,000 750,000 - 750,000 1 US For real-time multianalytes screening. It provides knowledge about targets and compounds of interestanalysis for HPVsin the context of the cell. The results obtained will allow the user to understand the biological processes Group 3 pathogens.. There are at least 11 researchers that could use thisoccuring, which will then add to the knowledge of targets and pathways of interest.equipment if it is equipped for infectious disease.3 E11-Liquid Nitrogen Tank 10,000 5,000 5,000 10,000 1 Malaysia For long-term storage of cells and samples1,210,000 1,205,000 5,000 1,210,0003 Prof. Dr. Hany Ariffin Cancer research in Malayasian 1 E20-AutoMACS Pro Cell Separator 250,000 230,000 - 230,000 1 Germanypopulation(UMCRI)(Miltenyi Biotec)This equipment will primarily be used for cell culturing purposes. Prior to the main step, proteins andvarious cells must first be purified and isolated for culturing purposes. This appliance enablespurification of mRNA and cDNA synthesis as well as genome related works. The cell separator alsofunctions as a stem cell purifier. as mentioned earlier, with the main use mostly associated with cellculture activities, probability of sharing this equipment is highly unlikely due to the existent risk ofcontamination by cancer cellsThe equipment is of low maintenance and requires no specific software torun on. Space is readily available currently for placement of the appliance inthe laboratory.4 Prof. Dr. Onn HajiHashimThe use of acute phasereactant proteins as cancerbiomarkers (UMPCR)2 E24-Pipettes 7,500 7,500 - 7,500 1 MalaysiaLiquid handling(Eppendorf)257,500 237,500 - 237,5001 E1-Protein Array Scanner 330,000 330,000 - 330,000 1 The scanner provides flexibility and reproducibility for a range of diverse This equipment is necessary to address the objectives of the proposed project. The new advance No issue with space and electric requirement. The image analysis software ismicroarray applications and format sizes. It is automated, high throughput, technology of protein microarray provides a wide variety of proteomics application, which includes: included with the whole system.and includes numbers of flexible features, including parallel dual-channel micro multi-analyte immunoassays, protein-antibody, antibody-protein, antibody-antigen, proteinprotein,and protein-drug microarray assays. The open system also allows acquisition of data anddetection and the ability to operate with a wide excitation range and a hugeselection of additional fluorescence emission filters. The scanner comes expression analysis from a wide range of gene array chips (whole human/mouse genome or specificwith inbuilt image analysis software, which supports its flexibility,group of genes). Analysis of multiple assays in a single run could greatly reduce the time consumption,automation and batch processing capabilities.number of sample and cost compared to conventional method. Protein Array also benefit from lessercross reactivity of antibody binding since each spot contains only single type of antibody. The systemwill be particularly useful for genomics and proteomics researchers within the Faculty of Medicine andUniversity of Malaya.5 Prof. Dr. Mohd RaisMustafaMolecular mechanisms ofdrug actions330,000 330,000 - 330,0001 E4-Real time PCR 200,000 200,000 - 200,000 1 USA Real-time PCR machine will be used to perform genes amplification which is Currently, 4 units is being purchased by FOM to cater to various research groups in variousThis equipment requires minimal maintenance and space is available at thea common method used in all molecular work for validation and real-time departments of FOM. In view of the increased in number of research groups and postgraduate students department for equipment placement.quantification of genes expression. This method is expected for any good in FOM. This number is still inadequate because one run takes 4 hours, which means in a day, at mostpublication and many researchers now have replaced the traditional semiquantitativethe equipment can do about 4 runs, hence, only 1 student from 1 research group can use 1 equipmentmethod with real-time quantitation.at a time, considering that the student will need the initial 2 runs to optimize amplificationparameters. In many experiments, usually a minimum of 5-10 genes are studied and this can alsoinvolves 100-200 patient samples such as that in pharmacogenomic studies. Hence, there is a need foran additional unit in Pharmacology department, not only to cater to the molecular work of this projectbut also to be used for other research groups in the department. Without this equipment, there will bea long queue to use the present number of equipment and this will delay the experiment and may alsojeopardize sensitive samples.200,000 200,000 - 200,000- updated on 2nd March 2012 -
HIR-MOHE EQUIPMENT 2012 - 2013 (FACULTY OF MEDICINE)No PI's Name Project Title EquipmentsEstimated BudgetYear 2 & 3(2012-2013)2012(RM)Approved Budget(After VM Lab)2013(RM)Total(RM)QuantityCountry ofOriginDetailsJustificationAdditional information(space/utilities eg electric, software etc)Potential users/researchgroupsFrequency andintensity of usage6 Assoc. Prof. Dr. Tunku Improving articular disease 1 E6-Motion capture systemKamarulprediction and management,regenerative therapy andsarcoma management2 E7-MIMICS Visual 3D software(NOCERAL)3 E8-Softwares (Sigma plot, SPSS, etc.,)250,000 320,000 - 320,00041,000 0 - -35,000 0 - -1 This instrument is required to quantify the baseline movement pattern. Data obtained will beinformative and used to determine the root cause of injuries and to develop a treatment plan.1 Required to process and edit the 2 D image data (CT, micro CT, MRI etc.,) to construct 3 D models withatmost accuracy and flexibility.1 Statitical softwares are required to analyse and validate the genereated experimental datas , makecomplex graphs and illustratrions for a better publication.4 E9-Inverted microscope5 E10-3D Surface Scanner20,425 20,500 - 20,500160,000 - 120,000 120,0001 To view live cells by allowing the entire environment upon which the organism resides on to themicroscope stage.1 Required to analyse a real-world object or environment to collect data on its shape and possibly itsappearance. Data obtained can be applied to design orthotecis and prostehetics for clinical use.6 E11-Mechanobiology station 81,000 - 81,000 81,000 1 A platform to conduct all experiments related to mechantransduction.7 E12-Portable cutting unit 30,000 - 30,000 30,000 1 Requried to cut the hard bones for biomechanical and histological analysis.8 E13-Shaking incubator40,000 - 40,000 40,0001 Required for mixing or biological liquids as wellas the incubation and cultivation of bilogical samples.9 E21-CO2 Incubator 14,025 - 14,100 14,100 1 To grow the cells in a temperature and Co2 controlled environment.671,450 340,500 285,100 625,6007 Prof. Dr. Awang Development of spatiotemporaldengue modelresearchers.1 E2-12 workstations 40,000 34,000 - 34,000 1 Taiwan Computer workstation to be used for modelling by doctoral students and Math modelling and computer simulation of models.Bulgiba&infectious disease meta- 2 E7-segmental body composition analyser 120,000 60,000 60,000 120,000 1 Japan Bio-electrical impedance analyser to be used for measuring body fat Measurement of body composition for modelling cardiovascular diseases.analysis (JULIUS)and water composition.Space has been made available. We do not have enough workstations at themoment .We need this to carry out surveys on body composition. We do not have anysuch analyser in JCUM at the present moment.3 E9-freezer 90,000 45,000 45,000 90,000 1 USA 80 degree below zero freezer fo storing biospecimens. Storage of frozen biomarkers. There is insufficient storage space in the current freezers. We will put thefreezer in a common space and will be used by all investigators involved inthis project.4 E10-LCo2 back up system 30,000 - - - 1 USA Liquid carbon dioxide system as backup for freezer. Backup system for frozen biomarker samples. This is a backup system for the freezers.280,000 139,000 105,000 244,0008 Dr. Puteri Shafinaz Shared Equipment for Faculty 1 E1-Quadrupole-TOF Mass spectrometer 2,850,000 2,750,000 100,000 2,850,000 1 USA The equipment is a hybrid of liquid/chip separation system and quadrupole The equipment is very crucial for the HIR projects that it will be supporting.A designated lab space is available in the Proteomics Facility, MedicalAkmar Bt Abdul (central Facility)TOF mass spectrometer. It is capable of performing high throughput Acquisition of accurate mass for identification of proteins, metabolites and characterization of PTM Biotechnology Laboratory, Faculty of Medicine which currently houses theRahmantandem MS analysis (scan MS/MS sensitivity and full MS3 capabilities). modification requires high quality data from mass spectrometry analysis and a comprehensive MALDI-TOF MS. The room has been equipped with two air-conditioning unitsDetection of protein, peptide and metabolites at low femtogram-level database is an integral part of these projects. This level of analysis can only be achieved with the for round the clock temperature control for MS equipment. Electrical voltagesensitivityavailability of the QTOF instrument and software package.supply to meet the requirement of the new MS will be arranged with UM’sDynamic range and mass sensitivity performance : 2ppm mass accuracy.electrical engineering unit.High speed data acquisition rate: 50 spectra/second for The Chip LC system The instrument proposed integrates HPLC Chip separation directly on-line to the high resolution QTOF Protein mass identification search engine, post-translational patternis ideal to study protein at post-transcriptional modification level where it system. This allows high speed spectral acquisition rates permitting in depth coverage of samples that prediction software and proprietary database for known glycoproteins andallows in-depth characterization of alteration of protein glycosylation and can be analyzed in a shorter experimental time compared to conventional LC spotter-MALDI. The chip- phosphoproteins are included with the purchase of equipment. In the tenderphosphorylation. The system also allows relative quantitative analysis of MS interface is specifically designed to enhance the LC-MS/MS workflow where it allows PTMwe will ensure that future software/database update will be provided at noproteins using isotope- labeled and non-labeled approaches.investigations which is presently the cutting-edge technology in proteomics research.cost for the next 3 years. Without this equipment, many research projectsWithout this equipment, many research projects will be delayed and this will impede the process of will be delayed and this will impede the process of high quality datahigh quality data acquisition that is needed for publication in Tier 1 journals inacquisition that is needed for publication in Tier 1 journals in proteomicsproteomics/metabolomics research.research.The system proposed is not specific to the needs of one group and can be used for a wide range ofapplication being proteomics/metabolomics/glycomics/lipidomics. It will also be able to support thegrowing number of users that are requesting for an alternative approach to move from gel-based toliquid-based proteomics platform.2 E2-High Definition Low Vacuum Scanning1,100,000 1,100,000 - 1,100,000 1 USA Scanning electron microscopy (SEM) is an important equipmentPresently, there is a high request from FOM users for SEM services : average 3-4 students/week. All the An allocated space (20' x 25', temparature 15-20°C, Humidity - 60% or less)Electron Microscopyin many multi-facet analysis involving cellular and extraSEM experiments and research done by the students have to use the equipment in other faculty or for installation of the unit is already available at the Electron Microscopycellular matrix interactionuniversities. The researcher now use the SEM at ISB and IMR- however there is very long queue, it is (EM) Unit, Faculty of Medicine. The equipment will be operated by trainedresponses. The proposed equipment does not require negative pressure or time consuming and not practical to users.EM unit staff.vacuum to produce images nor processing or gold for enhance imaging. EM unit has the pre-requisites needed for sample/slide preparation prior to the viewing procedureunder SEM. Each student will need 2 days for sample preparationThere are also specific requirements of samples for live cell imaging whereby the sample must beviewed at the same place as sample preparation for optimum condition.This system proposed is intended as shared equipment under Electron Microscopy Unit, FOM. It is notspecific to the needs to one group and can be used for a wide range of applications such as in the fieldsof:• Parasitology • Oncology Research • Microbiology • Infectious Disease • Virology• Anatomy•Neurology• Stem Cell Biology•Availability of SEM at FOM will help expedite the progress of research projects and acquisition of highquality images needed for Tier 1 publication in the above mentioned research areas3 E5- Micro-CT scanner/PET scanner 2,800,0002,800,000 -2,800,000 1 United Kingdom This instrument is required to study the small animal models of diseases or In vivo evaluation in a longitudinal study is only possible with the use of this equipment. It utilizes In terms of space wise, we have allocated a space in the newly renovatedinjuries seen in humans. It will also allow anatomical, structural, functional different physical principles or fundamentals to provide unique biological information from the Electron Microscopy lab in the FOM. For the first 2 years, we'll make sureand molecular imaging of underlying subject in vivo. It allows images of underlying subject. A large range of users can use this system, with interest in either live or cadaveric that the appointed vendor absorbs the costs for full maintenance into theradiograph of calcified tissuesmodels. It also allows other users to incorporate their in vivo studies to include more sophisticated initial price. We will hire a researcher with medical physics or radiographye.g. bones, teeth etc. or contrast injected models to be imaged and studied imaging and live cell tracking when implemented within the animal. Availability of such system background to run the day to day operations on the equipment. Besides that,in close details. Images can later be reconstructed in 2-D or 3-D images with definitely leads to increase in research productivity in the following research areas:in the tender later, it will clearly stated that the future software upgrades willor without contrast. It is safe from radiation and does not require any • Oncology Researchbe provided at no cost for 3 years.specific licensing.• Infectious DiseaseFurthermore it can also include whole animal studies.• Inflammation• Metabolic Diseases• Neurology• Gene Therapy• Stem cell Biology• Cardiovascular Disease• Immunology & Transplantation Biology• Toxicology• Drug Metabolism Studies.Currently, from our own findings, there is no similar piece of equipment available in UM. Hence, thisequipment will be part of a core animal imaging facility and will serve the imaging needs of investigatorsfrom UM and other institutes in greater Klang valley. Turning down the request will introduce somedelay in the establishment of animal imaging facility. Some projects which could only be leveraged bythe requested scanner will be affected.6,750,000 6,650,000 100,000 6,750,000- updated on 2nd March 2012 -
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