Peptide-Based Drug Design

46 Stegemann and Hoffmann
positive ion-mode. Note that in negative ion mode the [M-H] − is detected and
that in positive ion mode the [M+H] + signal at a 2 u higher mass is fragmented.
22. When several samples are processed in parallel it is not possible to mix the SACA
reagent with the reaction buffer to add the mixture to the dried peptide. In this
case add first 2 �L reaction buffer to each sample to dissolve the peptide and add
afterward 2 �L SACA reagent to each sample.
Acknowledgment
We thank Prof. Dr. Vladimir Kokryakov, Alexander Kolobov, and Ekaterina
Korableva for providing peptide fractions with antimicrobial activities for
sequence analysis. Financial support by the Deutsche Forschungsgemeinschaft
(DFG) and the European Regional Development Fund (EFRE, European Union,
and Free State Saxonia) is gratefully acknowledged.
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