Peptide-Based Drug Design

84 Giannakis et al.
(>20 kDa). Laser settings for the higher mass range need to be increased as the
larger molecules require more energy to “fly,” and hence these setting may not be
optimal for detection of smaller peptide fragments and yield off-scale readings.
10. Following the recommendations provided, a R 2 value of >0.95 is easily
obtainable, demonstrating the highly quantitative nature of the technology.
11. The aggregation and lipid-binding assays do not exhibit the same degree of selectivity/specificity
as the Ab capture experiments; hence these assays are useful for
the examination of less complex systems, including synthetic peptides and cell
culture supernatants.
12. During the lipid assay, A� binding time is kept to a minimum to prevent the
peptide further aggregating in the presence of the lipid.
5. Acknowledgments
EG and JDW gratefully acknowledge the Ian Potter Foundation (Australia)
for a grant for the establishment of a biomarker facility at the Howard Florey
Institute. DPS is a Wellcome Trust Travelling Fellow.
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