reSOLUTION_Research_09_Neuroscience - Leica Microsystems

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HigH content screening High Content Screening Automation for Confocal and Widefield Microscopes amplify the power of imaging Peter sendrowski, leica microsystems High content screening is a rapidly growing approach in life science research to answer complex questions in a shorter amount of time. as the paradigm shifts from descriptive imaging to quantitative analysis, researchers benefit from non-biased results using automated imaging systems to efficiently discover relationships between cells and organisms. fig. 1: High content screening with interactive system control for fully automated mitosis acquisition: microtubule secondary screen: scrambled srna tubulin (green), H2b (red). leica tcs sP5. objective: 63x oil (pre-scan); zoomed, maximum projection: 30 x 0.4 μm slices, 2 channels (high resolution). courtesy of christian conrad, embl, Heidelberg, germany. (1) mitocheck Project: www.mitocheck.org. 20 resolutioN
leica microsystems introduces a new automated concept to obtain imaging data: leica Hcs a High content screening automation. the combination of a high resolution confocal point scanner or widefield system with las af matrix m3 software offers valuable benefits for biomedical research: • the highest resolution provides maximum information and is the basis for precise analysis. • sophisticated automation of the imaging system enables far more experiments to be run in a shorter amount of time in a standardized way. statistically relevant and unbiased datasets are generated. • leica Hcs a provides flexibility and facilitates easy adaptation to the experiments of today and tomorrow. by adding las af matrix m3, the value of an imaging system becomes more than the sum of its parts. leica Hcs a speeds up experimental throughput and amplifies laboratory capacity. intelligent microscopy leica microsystems provides the technology to perfectly match the needs of researchers. from basic mosaic up to 3d or 4d time resolved multiwell analysis, extensive automation capability converts confocal and widefield systems into fully featured high content screening devices. five new autofocus routines plus z-drift compensation ensure that the target specimen remains in focus. thus, high quality data is generated even during long term measurements. automated tracking functions move even cells that escape during observ-ation back into the central imaging field. a variety of multiple scan jobs can be freely combined at multiple positions, which offers maximum freedom for new experiment designs. With this, leica Hcs a offers the flexibility to easily combine low resolution prescreens with high resolution secondary scans. HigH content screening fig. 2: leica Hcs a includes re-engineered mosaic algorithms for excellent results at the push of a button – to visualize the finest details as well as information overviews: mouse diaphragm muscle stained against neuro filament 150. mosaic: xyz: 5 x 5 x 101 images. (green: secondary antibody coupled to alexa fluor 488) and acetylcholine receptors (red: alphabungarotoxin coupled to alexafluor 647). courtesy of dr. rüdiger rudolf, cellular signaling in skeletal muscle, Karlsruhe institute of technology, germany. fig. 3: leica Hcs a supports frequently used multiwell plate formats to automatically study multi-dimensional experiments: time resolved or concentration dependent tests unveil true biological context. zebrafish, daniorerio, neurogenin - gfP. H2a courtesy of J. legradi, dr. u. liebel, Kit Karlsruhe institute of technology, germany. neuroscience 21
Page 1 and 2: customer magazine for neuroscience
Page 3 and 4: Leica TCS CARS Opens New Ways for R
Page 5 and 6: Making CARS Microscopy Accessible
Page 7 and 8: How is the new leica system used fo
Page 9 and 10: confocal microscoPy Leica TCS SP5 S
Page 11 and 12: Superresolution Opens up New Perspe
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Page 15 and 16: signal distance tcs sted, sub 60 nm
Page 17 and 18: New Insights Into the Dynamic Organ
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Page 23 and 24: Definiens Developer XD and Leica HC
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Page 29 and 30: Single-cell Analysis After Laser Mi
Page 31 and 32: neurons are just beginning to degen
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Page 35 and 36: New Tool to Study Traumatic Brain I
Page 37 and 38: Leica Vibratome Series cutting edge
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reSOLUTION_Research_09_Neuroscience - Leica Microsystems

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