Schriften zu Genetischen Ressourcen - Genres

O.Y. ANTONOVA, L.I. KOSTINA, T. GAVRILENKO, K. SCHÜLER and R. THIEME
Tab. 1: Comparison of fingerprints of cultivars long-term stored in two different
genebanks (+ = identical, - = different, / = not tested)
Cultivar Origin Year of
Release
Identical or different banding patterns by
using SSR primers (No)
1 2 3 4 5 7 9 10 12 13 15 16
Desiree Netherlands 1962 + + + + + + + + + + + +
Detskoselskiy
Russia 1959 + + + + + + + + + / + +
Dorisa Germany 1984 + + + + + + / + + + / +
Early Rose USA 1867 + + + + + − − − + − + −
Epicure Great Britain 1897 − − − − + − − − + − − −
Hilta Germany 1983 + / + + + + + + + / + +
Lorch Russia 1931 + + + + + + + + + / + +
Lugowskoy Ukraine 1987 + + + + + + + + + / + +
Naroch Belarus 1986 + + + + + + / + + + + +
Provita Netherlands 1967 + + + + + + + + + + + +
Switanok
kiewskiy
Ukraine 1987 + + + + + + + + / + + +
Sineglaska Russia - + + + + / + + + + + + +
Material and Methods
Twelve commercial potato cultivars, which are long-term stored in the genebanks of
Groß Lüsewitz and St. Petersburg were analysed (Tab. 1).
DNA was extracted from fresh-leaf tissue of greenhouse and in vitro grown plants
according to WIENAND and FEIX (1980). Twelve sets of primer sequences were used.
The source genes and SSR repeats are given in Table 2. PCR was performed as
described by KAWCHUK et al. (1996) with some modifications. The PCR products
were separated on non-denaturing polyacrylamide gels and silver stained according
to BUDOWLE and ALLEN (1993). The banding patterns were scored visually.
Results and Discussion
As expected for highly heterozygous, clonally propagated species like potato, PCRbased
markers are able to generate significant levels of DNA polymorphism to discriminate
genotypes. Using twelve pairs of SSR primers it was possible to distinguish
all the twelve cultivars, which have been stored up to 48 years separately in different
genebanks located at Groß Lüsewitz and St. Petersburg, respectively. Scorable
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