Schriften zu Genetischen Ressourcen - Genres

Proof of long-term stored potato germplasm by use of molecular markers
bands were counted for all genotypes. Identity of banding patterns between genebank
accessions was detected for ten of the twelve cultivars (Tab. 1, Fig. 1). However,
in the cultivars ‘Early Rose’ (Fig. 2) and ‘Epicure’, differences in banding patterns
were found for five and ten SSR primer pairs, respectively (Tab. 1, Fig. 3). The
following reasons are conceivable:
• Inclusion of donor cultivars carrying genetic modifications at the start of the collection.
• Occurrence of genetic changes during long-term storage caused by the use of
growth retardants or environmental stress in handling of in vitro and in situ collections.
• Instability of the cultivar.
• Mistakes or mixing of cultivars during the activities of the conservation methods.
Genotypes with different banding patterns should be further tested by comparison of
morphological traits according to the description of the cultivar list. Material of the
same cultivar should be ordered from a third genebank to compare the fingerprints.
First results showed that DNA fingerprinting with SSR primers is a useful, rapid and
reliable method to check cultivar identity in genebank collections. In further experiments
additional cultivars and other types of germplasm like wild potato species will
have to be included.
‘Desiree‘
a b
P1 P2 P5 P3 P4 P10 M
Fig. 1: Polyacrylamide gels depicting polymorphisms with different SSR
primer pairs (P) between the cultivars ‘Desiree’ and ‘Provita’ stored in
the genebanks of Groß Lüsewitz (a), and St. Petersburg (b), M=100
Base-Pair Ladder
194
a b
a b
a b
a b
a b
‘Provita‘
a b
a b
a b
a b
a b
a b
a b
a b
M P2 P4 P5 P1 P12 P13 P16 P3