TCSPC for FLIM and FRET in - Boston Electronics Corporation
TCSPC for FLIM and FRET in - Boston Electronics Corporation
TCSPC for FLIM and FRET in - Boston Electronics Corporation
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
<strong>FLIM</strong> Upgrade Kits <strong>for</strong> Confocal MicroscopesFluorescence lifetime imag<strong>in</strong>g with ps resolutionExcitation by picosecond diode laserConfocal detectionSimultaneous detection <strong>in</strong> several wavelength <strong>in</strong>tervalsMulti-dimensional <strong>TCSPC</strong> techniqueExcellent time-resolution <strong>and</strong> sensitivityZoom <strong>and</strong> image rotation functions of microscope applicableWorks at any scan rateSeparation of fluorophores by lifetimeAutofluorescence<strong>FRET</strong> experimentsImag<strong>in</strong>g of pH, ion concentrations, or oxygen concentrationThe systems are based on a picosecond diodelaser of 405 nm or 473 nm wavelength withhigh-efficiency s<strong>in</strong>gle-mode fibre coupl<strong>in</strong>g, as<strong>in</strong>gle-, dual-, or multi-wavelength detectormodule, <strong>and</strong> BH’s proprietary multi-dimensional<strong>TCSPC</strong> technique. The pulsed laser light is fed<strong>in</strong>to the microscope via a st<strong>and</strong>ard s<strong>in</strong>gle-modeoptical fibre. A high speed detector assembly isattached to a suitable optical output of the scanhead. Both directly coupled <strong>and</strong> fibre-coupledversions are available. A large variety of detectormodules is available <strong>for</strong> s<strong>in</strong>gle-wavelength, dualwavelength,or multispectral detection <strong>in</strong> 16wavelength channels. The signals of all detectorchannels are recorded simultaneously by multidimensional<strong>TCSPC</strong>, result<strong>in</strong>g <strong>in</strong> excellent efficiency<strong>and</strong> time resolution. Typical applicationsare multi-spectral autofluorescence imag<strong>in</strong>g oftissue, pH imag<strong>in</strong>g <strong>in</strong> tissue, comb<strong>in</strong>ed <strong>FLIM</strong><strong>and</strong> SHG imag<strong>in</strong>g, <strong>and</strong> <strong>in</strong>vestigation of prote<strong>in</strong><strong>in</strong>teraction by <strong>FRET</strong>.The laser is a jo<strong>in</strong>t product of BH <strong>and</strong> LASOS<strong>TCSPC</strong> technique covered by patents DE 43 39 784 <strong>and</strong> DE 43 39 787Becker & Hickl GmbHNahmitzer Damm 3012277 Berl<strong>in</strong>, GermanyTel. +49 30 787 56 32Fax +49 30 787 57 34<strong>in</strong>fo@becker-hickl.comwww.becker-hickl.comLASOS Lasertechnik GmbHCarl-Zeiss-Promenade 1007745 Jena, GermanyTel. +49 3641-2944-54Fax +49 3641 2944-17lasos<strong>in</strong>fo@lasos.comwww.lasos.comUK Representative:Photonic SolutionsPLCTel. +44 1316648122sales@psplc.comwww.psplc.comUS Representative:<strong>Boston</strong> <strong>Electronics</strong> Corp91 Boylston Street, Brookl<strong>in</strong>e.Massachusetts 02445 USATel: (800) 347 5445 or (617) 566 3821Fax: (617) 731 0935tcspc@boselec com, www.boselec.com
Change language